Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,011,464	T→C	I155V (ATC→GTC)	fabA ←	bifunctional 3‑hydroxydecanoyl‑ACP dehydratase/trans‑2‑decenoyl‑ACP isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,011,464	0	T	C	100.0%	40.5 / NA	12	I155V (ATC→GTC)	fabA	bifunctional 3‑hydroxydecanoyl‑ACP dehydratase/trans‑2‑decenoyl‑ACP isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/4);  total (8/4)

AACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGACCAGTGAAT  >  NZ_CP009273/1011358‑1011588                                                                                                           |                                                                                                                             aaCCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACtt                                                                                                       >  1:600/1‑130 (MQ=255) aaCCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACtt                                                                                                       <  2:600/130‑1 (MQ=255)    cTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCa                                                                                           <  1:215036/139‑1 (MQ=255)              cGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAAt                                                                                 >  1:93097/1‑139 (MQ=255)                    tttCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAgacg                                                                           <  2:31121/139‑1 (MQ=255)                                            aTTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGt                                                    <  2:167979/138‑1 (MQ=255)                                                              aCGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACttt                                 >  1:207637/1‑139 (MQ=255)                                                                             gACCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAg                  >  1:496872/1‑139 (MQ=255)                                                                              aCCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAg                  >  1:255250/1‑138 (MQ=255)                                                                              aCCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAg                  >  2:129950/1‑138 (MQ=255)                                                                              aCCGACTTTCAGGTCGCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAg                  >  2:546242/1‑138 (MQ=255)                                                                                             gCTGGCGGTATAGACCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGACCAGTGAAt  >  2:470487/1‑139 (MQ=255)                                                                                                           |                                                                                                                             AACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGACGGTTAACAATGCGTTTAAAGTGAATACGGTAGGTCACTTTTTTCGCTGTCGGCAGTACCTGACCAGTGAAT  >  NZ_CP009273/1011358‑1011588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑