Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,068,442	A→C	G323G (GGT→GGG)	rutA ←	pyrimidine utilization protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,068,442	0	A	C	100.0%	86.6 / NA	26	G323G (GGT→GGG)	rutA	pyrimidine utilization protein A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/14);  total (12/14)

TCATCATGCCACCTCCTGAGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCACGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTCAGTACCG  >  NZ_CP009273/1068316‑1068568                                                                                                                               |                                                                                                                               tcatcaTGCCACCTCCTGAGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCtt                                                                                                                    >  2:5546/1‑139 (MQ=255)      atGCCACCTCCTGAGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGAc                                                                                                               >  1:285564/1‑139 (MQ=255)                   aGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACa                                                                                                    <  1:276702/137‑1 (MQ=255)                       aGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACATgcgcg                                                                                              <  2:291418/139‑1 (MQ=255)                       aGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACATgcgcg                                                                                              >  2:225174/1‑139 (MQ=255)                       aGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACATgcgcg                                                                                              >  2:155047/1‑139 (MQ=255)                         cgcAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACATgcgcgc                                                                                             >  1:497915/1‑138 (MQ=255)                             ggTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACATGCGCGCGacac                                                                                        <  1:5546/139‑1 (MQ=255)                                      gcgcGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGt                                                                                  >  1:449922/1‑136 (MQ=255)                                        gcgGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACATGCGCGCGACACt                                                                                       <  2:96688/129‑1 (MQ=255)                                        gcgGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACATGCGCGCGACACt                                                                                       <  2:263049/129‑1 (MQ=255)                                        gcgGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACATGCGCGCGACACt                                                                                       >  1:96688/1‑129 (MQ=255)                                        gcgGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACATGCGCGCGACACt                                                                                       >  1:263049/1‑129 (MQ=255)                                                 cATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTaa                                                                     <  2:285564/138‑1 (MQ=255)                                                 cATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTaa                                                                     <  2:330873/138‑1 (MQ=255)                                                  aTCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACg                                                                   <  1:183983/139‑1 (MQ=255)                                                      gTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACATGCGCGCGACACt                                                                                       >  1:385840/1‑115 (MQ=255)                                                      gTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACATGCGCGCGACACt                                                                                       <  2:385840/115‑1 (MQ=255)                                                         gTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCa                                                             >  1:288643/1‑138 (MQ=255)                                                         gTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCa                                                             <  2:449922/138‑1 (MQ=255)                                                                 cgcTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTccc                                                               >  1:387639/1‑128 (MQ=255)                                                                 cgcTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTccc                                                               <  2:387639/128‑1 (MQ=255)                                                                                   aTTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGAt                                  <  2:311661/139‑1 (MQ=255)                                                                                                       gAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGtt               <  1:503369/138‑1 (MQ=255)                                                                                                       gAAGGTTAACAGCACGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGtt               <  2:288643/138‑1 (MQ=255)                                                                                                                     cGCCTTCGGCCCCAGGCACGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTCAGTACCg  >  2:382761/1‑137 (MQ=255)                                                                                                                               |                                                                                                                               TCATCATGCCACCTCCTGAGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCACGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTCAGTACCG  >  NZ_CP009273/1068316‑1068568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑