Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,079,189	T→C	S120P (TCC→CCC)	efeB →	iron uptake transporter deferrochelatase/peroxidase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,079,189	0	T	C	100.0%	56.0 / NA	17	S120P (TCC→CCC)	efeB	iron uptake transporter deferrochelatase/peroxidase subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/11);  total (6/11)

CCAGCGATAAAGCCGATCTTGAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATTCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAGCGCTTTGGCCTTGCGCCACAGATGCCAAAAAAGCTGCAGAAGATGACGCGTT  >  NZ_CP009273/1079073‑1079318                                                                                                                     |                                                                                                                                  ccAGCGATAAAGCCGATCTTGAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACa                                                                                                              <  2:466055/138‑1 (MQ=255)                 tCTTGAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAAtct                                                                                              >  1:247201/1‑138 (MQ=255)                          gttgttTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACg                                                                                    <  2:544361/139‑1 (MQ=255)                           ttgttTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGt                                                                                   >  1:112183/1‑139 (MQ=255)                             gtgtCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCTCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGtt                                                                                  <  2:165339/135‑1 (MQ=255)                                 cGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCg                                                                              <  2:333650/138‑1 (MQ=255)                                       ttgACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGt                                                                        <  1:307596/138‑1 (MQ=255)                                           cTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTc                                                                   <  2:112183/139‑1 (MQ=255)                                                       cTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTtgatg                                                        <  1:576090/138‑1 (MQ=255)                                                          ttCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAgcg                                                    >  2:318054/1‑139 (MQ=255)                                                                               caCCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAGCGCTTTGGCCTTGCGCCACAGAt                               >  1:274751/1‑139 (MQ=255)                                                                                         gCCAAATCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAGCGCTTTGGCCTTGCGCCACAGATGCCAAAAAAg                     <  2:274751/139‑1 (MQ=255)                                                                                            aaaTCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAGCGCTTTGGCCTTGCGCCACAGATGCCAAAAAAg                     <  1:476347/136‑1 (MQ=255)                                                                                            aaaTCCGCGCCTGCCACCACTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAGCGCTTTGGCCTTGCGCCACAGATGCCAAAAAAg                     >  2:476347/1‑136 (MQ=255)                                                                                                            ccacTCGATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAGCGCTTTGGCCTTGCGCCACAGATGCCAAAAAAGCTGCAGAAGATGACGCGtt  <  2:439352/139‑1 (MQ=255)                                                                                                                  gATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAGCGCTTTGGCCTTGCGCCACAGATGCCAAAAAAGCTGCAGAAGATGACGCGtt  >  1:331000/1‑133 (MQ=255)                                                                                                                  gATCCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAGCGCTTTGGCCTTGCGCCACAGATGCCAAAAAAGCTGCAGAAGATGACGCGtt  <  2:331000/133‑1 (MQ=255)                                                                                                                     |                                                                                                                                  CCAGCGATAAAGCCGATCTTGAGCGGTTGTTTCGCTTGTTGACTCAGCGTTTTGCTTTTCTGACTCAGGGCGGAGCAGCACCAGAAACGCCAAATCCGCGCCTGCCACCACTCGATTCCGGCATTCTTGGCGGCTACATTGCGCCCGATAATCTCACCATCACGTTATCGGTGGGTCACTCATTGTTTGATGAGCGCTTTGGCCTTGCGCCACAGATGCCAAAAAAGCTGCAGAAGATGACGCGTT  >  NZ_CP009273/1079073‑1079318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑