| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,079,811 | A→C | D327A (GAC→GCC) | efeB → | iron uptake transporter deferrochelatase/peroxidase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,079,811 | 0 | A | C | 100.0% | 35.2 / NA | 12 | D327A (GAC→GCC) | efeB | iron uptake transporter deferrochelatase/peroxidase subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (6/6); total (6/6) | |||||||||||
GCAGACGATTTTTGGCCGTGATAAGCAAACCGGTGCGCCGCTGGGAATGCAGCATGAGCATGATGTGCCTGATTACGCCAGCGACCCGGAAGGGAAGGTGATCGCGCTGGACAGCCATATCCGGCTGGCGAATCCCCGCACGGCGGAGAGTGAGTCCAGCCTGATGCTGCGTCGTGGCTACAGTTATTCACTGGGCGTCACCAACTCCGGGCAA > NZ_CP009273/1079701‑1079914 | gcagACGATTTTTGGCCGTGATAAGCAAACCGGTGCGCCGCTGGGAATGCAGCATGAGCATGATGTGCCTGATTACGCCAGCGACCCGGAAGGGAAGGTGATCGCGCTGGCCAGCCATATCCGGCTGGCGAATCCCCGc < 1:450073/139‑1 (MQ=255) ttGGCCGTGATAAGCAAACCGGTGCGCCGCTGGGAATGCAGCATGAGCATGATGTGCCTGATTACGCCAGCGACCCGGAAGGGAAGGTGATCGCGCTGGCCAGCCATATCCGGCTGGCGAATCCCCGCa > 1:414849/1‑129 (MQ=255) ttGGCCGTGATAAGCAAACCGGTGCGCCGCTGGGAATGCAGCATGAGCATGATGTGCCTGATTACGCCAGCGACCCGGAAGGGAAGGTGATCGCGCTGGCCAGCCATATCCGGCTGGCGAATCCCCGCa < 2:414849/129‑1 (MQ=255) catgagcatgaTGTGCCTGATTACGCCAGCGACCCGGAAGGGAAGGTGATCGCGCTGGCCAGCCATATCCGGCTGGCGAATCCCCGCACGGCGGAGAGTGAGTCCAGCCTGATGCTGCGTCGTGGCTACAGTTATTCAc < 2:567928/139‑1 (MQ=255) atgTGCCTGATTACGCCAGCGACCCGGAAGGGAAGGTGATCGCGCTGGCCAGCCATATCCGGCTGGCGAATCCCCGCACGGCGGAGAGTGAGTCCAGCCTGATGCTGCGTCGTGGCTACAGTTATTCACTGGGCGTCAc > 1:117499/1‑139 (MQ=255) atgTGCCTGATTACGCCAGCGACCCGGAAGGGAAGGTGATCGCGCTGGCCAGCCATATCCGGCTGGCGAATCCCCGCACGGCGGAGAGTGAGTCCAGCCTGATGCTGCGTCGTGGCTACAGTTATTCACTGGGCGTCAc > 1:282771/1‑139 (MQ=255) ccTGATTACGCCAGCGACCCGGAAGGGAAGGTGATCGCGCTGGCCAGCCATATCCGGCTGGCGAATCCCCGCACGGCGGAGAGTGAGTCCAGCCTGATGCTGCGTCGTGGCTa < 1:468861/113‑1 (MQ=255) ccTGATTACGCCAGCGACCCGGAAGGGAAGGTGATCGCGCTGGCCAGCCATATCCGGCTGGCGAATCCCCGCACGGCGGAGAGTGAGTCCAGCCTGATGCTGCGTCGTGGCTa > 2:468861/1‑113 (MQ=255) ccTGATTACGCCAGCGACCCGGAAGGGAAGGTGATCGCGCTGGCCAGCCATATCCGGCTGGCGAATCCCCGCACGGCGGAGAGTGAGTCCAGCCTGATGCTGCGTCGTGGCTACAGTTATTCACTGGGCGTCACCAACt > 2:216892/1‑139 (MQ=255) cGCCAGCGACCCGGAAGGGAAGGTGATCGCGCTGGCCAGCCATATCCGGCTGGCGAATCCCCGCACGGCGGAGAGTGAGTCCAGCCTGATGCTGCGTCGTGGCTACAGTTATTCACTGGGCGTCACCAACTCCGGGCaa < 2:347360/139‑1 (MQ=255) aaggTGATCGCGCTGGCCAGCCATATCCGGCTGGCGAATCCCCGCACGGCGGAGAGTGAGTCCAGcc < 1:73418/67‑1 (MQ=255) aaggTGATCGCGCTGGCCAGCCATATCCGGCTGGCGAATCCCCGCACGGCGGAGAGTGAGTCCAGcc > 2:73418/1‑67 (MQ=255) | GCAGACGATTTTTGGCCGTGATAAGCAAACCGGTGCGCCGCTGGGAATGCAGCATGAGCATGATGTGCCTGATTACGCCAGCGACCCGGAAGGGAAGGTGATCGCGCTGGACAGCCATATCCGGCTGGCGAATCCCCGCACGGCGGAGAGTGAGTCCAGCCTGATGCTGCGTCGTGGCTACAGTTATTCACTGGGCGTCACCAACTCCGGGCAA > NZ_CP009273/1079701‑1079914 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |