Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,097,764	A→G	N78N (AAT→AAC)	csgE ←	curli production assembly/transport protein CsgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,097,764	0	A	G	100.0%	115.4 / NA	31	N78N (AAT→AAC)	csgE	curli production assembly/transport protein CsgE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/15);  total (16/15)

TCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGA  >  NZ_CP009273/1097630‑1097886                                                                                                                                       |                                                                                                                           tCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGa                                                                                                                        <  2:165916/139‑1 (MQ=255)                  cGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAg                                                                                                       >  2:306891/1‑139 (MQ=255)                  cGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAg                                                                                                       >  2:50869/1‑139 (MQ=255)                    ccTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGAc                                                                                                                       <  2:332645/121‑1 (MQ=255)                    ccTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGAc                                                                                                                       >  1:332645/1‑121 (MQ=255)                        aTTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACTTCCTGGTTGACCGTTATAGTGATCCAGCTTcc                                                                                                  <  1:507211/138‑1 (MQ=255)                        aTTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTccc                                                                                                 >  1:134179/1‑139 (MQ=255)                           tATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCa                                                                                               <  1:430429/138‑1 (MQ=255)                             tCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATc                                                                                             <  1:29069/138‑1 (MQ=255)                             tCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCg                                                                                            >  2:447518/1‑139 (MQ=255)                             tCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCg                                                                                            >  2:444086/1‑139 (MQ=255)                                               cttcttCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCAt                                                                           <  1:425362/138‑1 (MQ=255)                                                           gAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGt                                                               <  2:251493/138‑1 (MQ=255)                                                                  tGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGta                                                       >  2:177186/1‑139 (MQ=255)                                                                       aGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGt                                                               >  2:282905/1‑126 (MQ=255)                                                                       aGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGt                                                               <  1:282905/126‑1 (MQ=255)                                                                       aGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGt                                                   >  1:43296/1‑138 (MQ=255)                                                                            acAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACttt                                             >  1:459646/1‑139 (MQ=255)                                                                            acAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACttt                                             >  1:542346/1‑139 (MQ=255)                                                                            acAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACttt                                             >  2:57376/1‑139 (MQ=255)                                                                                   tctcGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTtata                                                                                                                >  1:44086/1‑65 (MQ=255)                                                                                   tctcGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTtata                                                                                                                <  2:44086/65‑1 (MQ=255)                                                                                       gAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTAt                                    <  2:542346/137‑1 (MQ=255)                                                                                                 ttCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCt                         <  2:113462/138‑1 (MQ=255)                                                                                                      cGGAAATAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTaa                   <  1:306891/139‑1 (MQ=255)                                                                                                           aTAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAAt               >  1:290146/1‑138 (MQ=255)                                                                                                            tAAAAAAGTCTGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCa             <  1:50869/139‑1 (MQ=255)                                                                                                                      tGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCaa      >  1:225219/1‑136 (MQ=255)                                                                                                                      tGGAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCaa      >  1:161643/1‑136 (MQ=255)                                                                                                                        gAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGa  <  1:57376/138‑1 (MQ=255)                                                                                                                        gAAAATAACGTCCTGGTTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGa  <  1:444086/138‑1 (MQ=255)                                                                                                                                       |                                                                                                                           TCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGTGCAAAGACGACAGTTTTCTCGAAGTCTCTTTTCAACGGAAATAAAAAAGTCTGGAAAATAACGTCCTGATTGACCGTTATAGTGATCCAGCTTCCCCATCGTGCACTGGGCCTTTCATTAATCGTTAAGTTACCCGTATAGTCACTTTCCCATTTATCACTAAAGGCTCGGTAAAAATCATGGCCAATAGA  >  NZ_CP009273/1097630‑1097886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑