Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	204,632	T→C	V1007A (GTC→GCC)	dnaE →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	204,632	0	T	C	100.0%	75.8 / NA	22	V1007A (GTC→GCC)	dnaE	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/10);  total (12/10)

ACCCTATCAACCAGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTATCG  >  NZ_CP009273/204503‑204760                                                                                                                                  |                                                                                                                                 acCCTATCAACCAGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAgcg                                                                                                                         <  2:495140/139‑1 (MQ=255)             aGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTAt                                                                                                             >  2:47773/1‑139 (MQ=255)             aGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTAt                                                                                                             >  2:304853/1‑139 (MQ=255)              gTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATc                                                                                                            <  1:422796/139‑1 (MQ=255)               tATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCg                                                                                                           >  1:361994/1‑139 (MQ=255)                 tttAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCgg                                                                                                          >  1:154371/1‑138 (MQ=255)                              gAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTg                                                                                             <  1:329640/138‑1 (MQ=255)                                           gAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCgg                                                                               >  1:118399/1‑139 (MQ=255)                                            aGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCggg                                                                              <  1:304853/139‑1 (MQ=255)                                            aGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCggg                                                                              <  1:47773/139‑1 (MQ=255)                                                 tAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCt                                                                         >  2:88854/1‑139 (MQ=255)                                                                            aaCGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCt                                              >  2:142198/1‑139 (MQ=255)                                                                            aaCGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCt                                              <  2:361994/139‑1 (MQ=255)                                                                                 ggTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGAt                                          >  1:576658/1‑138 (MQ=255)                                                                                  gTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATa                                         <  1:483533/138‑1 (MQ=255)                                                                                      aGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATAc                                    >  1:309856/1‑139 (MQ=255)                                                                                                 cTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGccc                                               <  1:533396/117‑1 (MQ=255)                                                                                                 cTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGccc                                               >  2:533396/1‑117 (MQ=255)                                                                                                       ggCTCGTTGTTGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGaaaa                   <  2:576658/139‑1 (MQ=255)                                                                                                            gttgttGCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGa                <  1:27424/137‑1 (MQ=255)                                                                                                            acgattgCCGCGCGGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGAcc              >  2:305737/5‑139 (MQ=255)                                                                                                                        cgGGTTATGGCCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTATCg  >  2:114726/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                 ACCCTATCAACCAGTATTTAAAAGAGATTGAGCGTTATGTCGGAGGCGTAAGGCTGAAAGACATGCACCCGACAGAACGTGGTAAAGTCATCACGGCTGCGGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTATCG  >  NZ_CP009273/204503‑204760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑