Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,136,234 A→G E249G (GAA→GGA)  flgL → flagellar hook‑associated protein FlgL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,136,2340AG100.0% 80.7 / NA 23E249G (GAA→GGA) flgLflagellar hook‑associated protein FlgL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/11);  total (12/11)

TGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATGCA  >  NZ_CP009273/1136106‑1136366
                                                                                                                                |                                                                                                                                    
tGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCACg                                                                                                                            >  1:449236/1‑139 (MQ=255)
tGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCACg                                                                                                                            >  1:353880/1‑139 (MQ=255)
  ccATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCACg                                                                                                                            >  1:343962/1‑137 (MQ=255)
                aaaaCGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTg                                                                                                             <  1:450650/138‑1 (MQ=255)
                         gTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTgg                                                                                                   <  1:181800/139‑1 (MQ=255)
                                    cGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAgg                                                                                         >  1:356848/1‑138 (MQ=255)
                                                                 cgTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACgcagca                                                           <  2:356848/139‑1 (MQ=255)
                                                                       aTAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAg                                                     <  1:173689/139‑1 (MQ=255)
                                                                                    cgGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGAt                                        <  1:342825/139‑1 (MQ=255)
                                                                                                         cAATGTGCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTAtt                   <  1:29941/139‑1 (MQ=255)
                                                                                                         cAATGTGCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTAtt                   <  2:191897/139‑1 (MQ=255)
                                                                                                         cAATGTGCTGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTAtt                   <  2:343962/139‑1 (MQ=255)
                                                                                                                 tGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTgg                                                                                                   <  1:2391/51‑1 (MQ=255)
                                                                                                                 tGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTgg                                                                                                   >  2:2391/1‑51 (MQ=255)
                                                                                                                 tGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGAtgtg                                     >  2:241857/1‑113 (MQ=255)
                                                                                                                 tGACTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTa           >  1:369146/1‑139 (MQ=255)
                                                                                                                 tGACTGTTCGCGCGGGATTAGGCACGAAGAGGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGAtgtg                                     <  1:241857/113‑1 (MQ=255)
                                                                                                                   aCTGTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTAca         >  2:287793/1‑139 (MQ=255)
                                                                                                                      gTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTAcatca      >  2:348481/1‑139 (MQ=255)
                                                                                                                      gTTCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTAcatca      >  1:323438/1‑139 (MQ=255)
                                                                                                                        tCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATg    >  2:224186/1‑139 (MQ=255)
                                                                                                                        tCGCGCGGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATg    >  2:317319/1‑139 (MQ=255)
                                                                                                                          gcgcgGGATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATgca  <  2:348066/139‑1 (MQ=255)
                                                                                                                                |                                                                                                                                    
TGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATGCA  >  NZ_CP009273/1136106‑1136366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑