Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,149,506	T→C	S251P (TCC→CCC)	pabC →	aminodeoxychorismate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,149,506	0	T	C	100.0%	54.8 / NA	17	S251P (TCC→CCC)	pabC	aminodeoxychorismate lyase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/9);  total (8/9)

GTTTGCTGGCACAATCCTCTTATCAGCTTGTCGAAGTGCAAGCCTCTCTGGAAGAGTCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCG  >  NZ_CP009273/1149375‑1149634                                                                                                                                    |                                                                                                                                 gTTTGCTGGCACAATCCTCTTATCAGCTTGTCGAAGTGCAAGCCTCTCTGGAAGAGTCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTc                                                                                                                           <  1:401366/139‑1 (MQ=255)                         aGCTTGTCGAAGTGCAAGCCTCTCTGGAAGAGTCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATAtt                                                                                                    <  1:211874/138‑1 (MQ=255)                         aGCTTGTCGAAGTGCAAGCCTCTCTGGAAGAGTCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATAtt                                                                                                    <  1:148273/138‑1 (MQ=255)                           cTTGTCGAAGTGCAAGCCTCTCTGGAAGAGTCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAg                                                                                                 >  1:217182/1‑139 (MQ=255)                                   aGTGCAAGCCTCTCTGGAAGAGTCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACtt                                                                                         >  1:496980/1‑139 (MQ=255)                                            ctctctGGAAGAGTCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAgcgc                                                                                <  2:361095/139‑1 (MQ=255)                                                   gAAGAGTCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAAt                                                                          <  1:582012/138‑1 (MQ=255)                                                         tCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCAt                                                                   >  2:465210/1‑139 (MQ=255)                                                               caggcagATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGaaaa                                                              <  2:496980/138‑1 (MQ=255)                                                                aggcagATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGaaaaaa                                                            <  2:217182/139‑1 (MQ=255)                                                                       tgagatgGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTAt                                                     >  1:350767/1‑139 (MQ=255)                                                                              gTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAAt                                              <  2:350767/139‑1 (MQ=255)                                                                                 aTTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCtt                                           >  1:264738/1‑139 (MQ=255)                                                                                                aTGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCtggtgg                             >  2:34626/1‑138 (MQ=255)                                                                                                 tGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTa                           <  1:465210/139‑1 (MQ=255)                                                                                                     aGTGATGCCCGTATGTGCCTGTGGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTgg                       >  2:312614/1‑139 (MQ=255)                                                                                                                          tgGCGATGTCCCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCg  >  2:354688/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                 GTTTGCTGGCACAATCCTCTTATCAGCTTGTCGAAGTGCAAGCCTCTCTGGAAGAGTCGTTGCAGGCAGATGAGATGGTTATTTGTAATGCGTTAATGCCAGTGATGCCCGTATGTGCCTGTGGCGATGTCTCCTTTTCGTCAGCAACGTTATATGAATATTTAGCCCCACTTTGTGAGCGCCCGAATTAGTCATGAAAAAAGTGTTATTGATAATCTTGTTATTGCTGGTGGTACTGGGTATCGCCGCTGGTGTGGGCG  >  NZ_CP009273/1149375‑1149634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑