| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,182,954 | T→A | D248V (GAT→GTT) | roxA ← | [50S ribosomal protein L16]‑arginine 3‑hydroxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,182,954 | 0 | T | A | 100.0% | 66.9 / NA | 19 | D248V (GAT→GTT) | roxA | [50S ribosomal protein L16]‑arginine 3‑hydroxylase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (10/9); total (10/9) | |||||||||||
AGGCGGCTCTGGCGGCGCGATATCCAGTTCATGACGTGACTGGGATATAAACTCGCCAAACCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTATCCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATCGCTGTAGTAGTTGCCGCCCAGTTCACGTTGCAGCACATAATCGGCAAATCCACTAATCAGTTCCCGCGTATTTGGCGCG > NZ_CP009273/1182836‑1183088 | aGGCGGCTCTGGCGGCGCGATATCCAGTTCATGACGTGACTGGGATATAAACTCGCCAAACCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAAc < 2:514247/138‑1 (MQ=255) ggcgCGATATCCAGTTCATGACGTGACTGGGATATAAACTCGCCAAACCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAg < 1:179043/139‑1 (MQ=255) cgcgATATCCAGTTCATGACGTGACTGGGATATAAACTCGCCAAACCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAgcg < 2:352659/139‑1 (MQ=255) gcgATATCCAGTTCATGACGTGACTGGGATATAAACTCGCCAAACCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACa < 2:345395/123‑1 (MQ=255) gcgATATCCAGTTCATGACGTGACTGGGATATAAACTCGCCAAACCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACa > 1:345395/1‑123 (MQ=255) atatCCAGTTCATGACGTGACTGGGATATAAACTCGCCAAACCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAg < 2:92372/139‑1 (MQ=255) ttCATGACGTGACTGGGATATAAACTCGCCAAACCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGTATGAGCGCGAGGTGGAACa > 1:241112/1‑139 (MQ=255) aTGACGTGACTGGGATATAAACTCGCCAAACCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATcc > 1:247582/1‑139 (MQ=255) gACGTGACTGGGATATAAACTCGCCAAACCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCg > 2:169430/1‑138 (MQ=255) cGTGACTGGGATATAAACTCGCCAATCCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGAt < 2:176489/139‑1 (MQ=255) gCCAAACCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATCGCTGTAGTAGTTgccgcc > 1:200969/1‑138 (MQ=255) ccATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATCGCTGTAGTAGTTGCCGCCCAGTTCa < 1:169430/139‑1 (MQ=255) ggTTGATCAATTCGAGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTgg > 1:82281/1‑79 (MQ=255) ggTTGATCAATTCGAGCAGCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTgg < 2:82281/79‑1 (MQ=255) aGCATCATCTCACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATCGCTGTAGTAGTTGCCGCCCAGTTCACGTTGCAGCACATAATCGGCAAATCCACTAATCAGtt < 2:197000/139‑1 (MQ=255) tctcACGCAGTTTAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATCGCTGTAGTAGTTGCCGCCCAGTTCACGTTGCAGCACATAATCGGCAAATCCACTAATCAGTTCCcgcg > 1:58097/1‑138 (MQ=255) tttAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATCGCTGTAGTAGTTGCCGCCCAGTTCACGTTGCAGCACATAATCGGCAAATCCACTAATCAGTTCCCGCGTATTTGgcgcg > 1:379380/1‑139 (MQ=255) tttAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATCGCTGTAGTAGTTGCCGCCCAGTTCACGTTGCAGCACATAATCGGCAAATCCACTAATCAGTTCCCGCGTATTTGgcgcg > 1:500490/1‑139 (MQ=255) tttAACCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATCGCTGTAGTAGTTGCCGCCCAGTTCACGTTGCAGCACATAATCGGCAAATCCACTAATCAGTTCCCGCGTATTTGgcgcg > 2:306316/1‑139 (MQ=255) | AGGCGGCTCTGGCGGCGCGATATCCAGTTCATGACGTGACTGGGATATAAACTCGCCAAACCATTGCTTAAAGTGTTCCGGCTGGTTGATCAATTCGAGCATCATCTCACGCAGTTTATCCATCTCTTGCGGCAGAACATCCGCAGGATGAGCGCGAGGTGGAACATCCGGATCGCTGTAGTAGTTGCCGCCCAGTTCACGTTGCAGCACATAATCGGCAAATCCACTAATCAGTTCCCGCGTATTTGGCGCG > NZ_CP009273/1182836‑1183088 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |