Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,220,495	T→A	D108V (GAT→GTT)	minD ←	septum site‑determining protein MinD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,220,495	0	T	A	100.0%	101.9 / NA	26	D108V (GAT→GTT)	minD	septum site‑determining protein MinD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (12/14);  total (12/14)

CGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAA  >  NZ_CP009273/1220366‑1220627                                                                                                                                  |                                                                                                                                     cgTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGaa                                                                                                                             >  1:142761/1‑139 (MQ=255)        aggagACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGc                                                                                                                      <  1:268419/139‑1 (MQ=255)           agaCTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGAc                                                                                                                   <  2:142761/139‑1 (MQ=255)           agaCTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGAc                                                                                                                   <  1:514074/139‑1 (MQ=255)                    ggTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGAc                                                                                                                   >  1:173136/1‑130 (MQ=255)                    ggTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGAc                                                                                                                   <  2:173136/130‑1 (MQ=255)                       tggtggTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGt                                                                                                       >  2:367640/1‑139 (MQ=255)                          tggtAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAg                                                                                                    <  1:515063/139‑1 (MQ=255)                              aataatGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCa                                                                                                >  1:165818/1‑139 (MQ=255)                                    ggCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCa                                                                                                            >  1:27132/1‑121 (MQ=255)                                    ggCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCa                                                                                                            <  2:27132/121‑1 (MQ=255)                                    ggCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTa                                                                                          >  1:258901/1‑139 (MQ=255)                                                   aTAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGAt                                                                           <  1:367640/139‑1 (MQ=255)                                                       agTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCg                                                                       >  2:479783/1‑139 (MQ=255)                                                                      ccGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGAt                                                        >  2:467809/1‑139 (MQ=255)                                                                                    tGCCGGGGAGTCACAAACGATAAATTCGAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCt                                            <  1:548719/137‑1 (MQ=255)                                                                                    tGCCGGGGAGTCACAAACGATAAATTCGAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCt                                            >  2:548719/1‑137 (MQ=255)                                                                                                  aaaCGATAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGc                            <  1:367429/139‑1 (MQ=255)                                                                                                        tAAATTCAAAATCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGAtt                      <  2:314066/139‑1 (MQ=255)                                                                                                                aaaTCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTg              >  1:35272/1‑139 (MQ=255)                                                                                                                aaaTCCATCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTg              >  2:257789/1‑139 (MQ=255)                                                                                                                      aTCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCa            >  2:573271/1‑135 (MQ=255)                                                                                                                      aTCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCa            <  1:573271/135‑1 (MQ=255)                                                                                                                       tCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGcc       <  2:15749/139‑1 (MQ=255)                                                                                                                       tCGCTTTCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGcc       <  1:471648/139‑1 (MQ=255)                                                                                                                             tCAGAACATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGaa  <  2:35272/138‑1 (MQ=255)                                                                                                                                  |                                                                                                                                     CGTACTGAGGAGACTTCCGGGTTGGTGGTAATAATGGCTTCGTCTGCAAAATAGAGTGCCATTAACGCACCGGTTTCAATCCCTGCCGGGGAGTCACAAACGATAAATTCAAAATCCATCGCTTTCAGATCATCAAGAACTTTGGCGACCCCTTCACGGGTGAGGGCATCTTTATCGCGTGTTTGCGATGCCGGCAGAATATAGAGATTTTCAGTACGCTTATCTTTAATTAACGCCTGATTTAGCGTTGCATCGCCCTGAA  >  NZ_CP009273/1220366‑1220627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑