| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,235,908 | T→G | A540A (GCA→GCC) | cvrA ← | potassium/proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,235,908 | 0 | T | G | 92.9% | 38.2 / NA | 14 | A540A (GCA→GCC) | cvrA | potassium/proton antiporter |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (5/8); minor base C (0/1); total (5/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCAC > NZ_CP009273/1235784‑1236038 | aTTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTaacaa > 1:33625/1‑139 (MQ=255) cTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCAcc < 1:550681/139‑1 (MQ=255) tACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCa > 2:190577/1‑139 (MQ=255) cacTGCATTATCTTCTTTCTCGGCCACCGTCCAGTTCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCg < 2:136216/139‑1 (MQ=255) ttATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCa < 2:372604/139‑1 (MQ=255) tcttctTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAGCCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAgg < 1:28576/138‑1 (MQ=255) tctttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCa > 2:479720/1‑138 (MQ=255) ctcGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCatatat < 1:560025/138‑1 (MQ=255) gCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAgcg < 1:556380/139‑1 (MQ=255) cGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACa < 2:280125/139‑1 (MQ=255) aGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGc < 1:479720/139‑1 (MQ=255) cccGGCAAACTCTACCTGGTCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTg > 1:260046/1‑139 (MQ=255) tCACCGACAACCGGGGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATg > 1:50107/1‑138 (MQ=255) aCAACCGGCGCCGCGCCTAACAACTGCTTGACAATTTCACCCATCGTTTGCTGCTTATCACGATATTCTCGCCCGTCCTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCAc < 2:252547/139‑1 (MQ=255) | ATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCAC > NZ_CP009273/1235784‑1236038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |