Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,251,036	T→G	T125P (ACA→CCA)	ycgV ←	autotransporter outer membrane beta‑barrel domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,251,036	0	T	G	100.0%	147.5 / NA	40	T125P (ACA→CCA)	ycgV	autotransporter outer membrane beta‑barrel domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (19/21);  total (19/21)

TTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCT  >  NZ_CP009273/1250901‑1251163                                                                                                                                        |                                                                                                                                ttAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAg                                                                                                                              <  2:361505/139‑1 (MQ=255)       ttGAGCTATGCCCAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGtt                                                                                                                        <  1:554847/139‑1 (MQ=255)       ttGAGCTATGCCCAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGt                                                                                                                         >  2:118729/1‑138 (MQ=255)          aGCTATGCCCAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGc                                                                                                                     <  2:392088/139‑1 (MQ=255)               tGCCCAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGt                                                                                                                  >  2:97417/1‑137 (MQ=255)               tGCCCAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGt                                                                                                                  >  1:450204/1‑137 (MQ=255)                 cccAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAgg                                                                                                              >  2:121724/1‑139 (MQ=255)                   cAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGa                                                                                                            <  2:357380/139‑1 (MQ=255)                     ataatGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGc                                                                                                          <  2:536322/139‑1 (MQ=255)                     ataatGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGc                                                                                                          <  1:121724/139‑1 (MQ=255)                             gCCGTCATCGTTCCTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGttttgg                                                                                                  >  2:337992/1‑137 (MQ=255)                               cGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGcc                                                                                                <  2:578046/139‑1 (MQ=255)                               cGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGcc                                                                                                <  2:423293/139‑1 (MQ=255)                                                 tGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCttt                                                                              <  1:75304/139‑1 (MQ=255)                                                     gctgcCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGCTGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCa                                                                          >  2:94706/1‑139 (MQ=255)                                                     gctgcCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCa                                                                          >  2:480607/1‑139 (MQ=255)                                                             tgCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCa                                                                    >  1:77726/1‑137 (MQ=255)                                                               ccTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCagag                                                                 <  2:77726/138‑1 (MQ=255)                                                                         cATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGcc                                                                                                >  2:350457/1‑97 (MQ=255)                                                                         cATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGcc                                                                                                <  1:350457/97‑1 (MQ=255)                                                                         cATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGa                                                       >  2:47889/1‑138 (MQ=255)                                                                                   gTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGt                                             >  2:218605/1‑138 (MQ=255)                                                                                    tCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGa                                           <  1:374382/139‑1 (MQ=255)                                                                                    tCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGa                                           <  1:337992/139‑1 (MQ=255)                                                                                      aCCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGAca                                         >  2:553395/1‑139 (MQ=255)                                                                                      aCCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGAca                                         >  1:547158/1‑139 (MQ=255)                                                                                      aCCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGAca                                         >  1:10004/1‑139 (MQ=255)                                                                                      aCCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGAca                                         >  2:427904/1‑139 (MQ=255)                                                                                       ccAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGAcat                                        >  1:407538/1‑139 (MQ=255)                                                                                        cAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTc                                                                                                                 >  2:144016/1‑65 (MQ=255)                                                                                        cAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTc                                                                                                                 <  1:144016/65‑1 (MQ=255)                                                                                         aaTTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGAcatca                                      <  2:450204/139‑1 (MQ=255)                                                                                                       cGATGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGat                        <  1:446964/139‑1 (MQ=255)                                                                                                          tGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGa                                           <  1:553826/117‑1 (MQ=255)                                                                                                          tGGCAGAGGTTTGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGa                                           >  2:553826/1‑117 (MQ=255)                                                                                                                     tGACCAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCgtgt          >  1:122708/1‑139 (MQ=255)                                                                                                                        ccAACAACATCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTc       <  2:407538/139‑1 (MQ=255)                                                                                                                             aacaTCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCctgct  <  2:122708/139‑1 (MQ=255)                                                                                                                             aacaTCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCctgct  <  2:10004/139‑1 (MQ=255)                                                                                                                             aacaTCTACTGGGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCctgct  <  1:115121/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                TTAGTGTTGAGCTATGCCCAATAATGATGCCGTCATCGTTACTCTTAATGGTGCTGCCTGTGCCTAAGTCAGCATGGGTATAGTCACCAATTAAGTTAATGCCGATGGCAGAGGTTTGACCAACAACATCTACTGTGAGTCGGTTAGCTGTCAGGGAGCTGTTTTTGCCCTGAATAATGATTCCTTTTGCATATCCAGAGGCGTCGTTGACATTGACGGTGACATCATTACCCAGGTTGATATTGCCAGGCGTGTTTCCTGCT  >  NZ_CP009273/1250901‑1251163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑