Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,306,087	A→G	intergenic (+22/+18)	tonB → / ← yciA	TonB system transport protein TonB/acyl‑CoA thioester hydrolase YciA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,306,087	0	A	G	100.0%	63.1 / NA	19	intergenic (+22/+18)	tonB/yciA	TonB system transport protein TonB/acyl‑CoA thioester hydrolase YciA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/10);  total (9/10)

GTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCC  >  NZ_CP009273/1305961‑1306220                                                                                                                               |                                                                                                                                      gTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGCCGGAGGCtttt                                                                                                                           <  1:342003/139‑1 (MQ=255)                                     ggTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGCCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCt                                                                                       >  1:222329/1‑139 (MQ=255)                                               gCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGCCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCaa                                                                             >  1:37011/1‑139 (MQ=255)                                                     ggATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGCCGGAGGCTTTTGACTATTACTCaa                                                                                                              >  1:131299/1‑100 (MQ=255)                                                     ggATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGCCGGAGGCTTTTGACTATTACTCaa                                                                                                              <  2:131299/100‑1 (MQ=255)                                                       aTTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGCCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACa                                                                     <  1:19166/139‑1 (MQ=255)                                                         tgtgGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGCCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACa                                                                     <  2:121286/137‑1 (MQ=255)                                                         tgtgGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGCCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACa                                                                     >  1:121286/1‑137 (MQ=255)                                                                aaTATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGCCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGa                                                                       >  2:63844/1‑128 (MQ=255)                                                                aaTATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGCCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGa                                                                       <  1:63844/128‑1 (MQ=255)                                                                aaTATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGCCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTaaa                                                             >  1:71853/1‑138 (MQ=255)                                                                        gTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGCCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTc                                                    >  1:400276/1‑139 (MQ=255)                                                                             aaaTTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGCCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCg                                               <  1:351162/139‑1 (MQ=255)                                                                             aaaTTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGCCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCg                                               <  1:319208/139‑1 (MQ=255)                                                                                   aCGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGCCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACcgcg                                                                        <  1:479511/108‑1 (MQ=255)                                                                                   aCGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGCCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACcgcg                                                                        >  2:479511/1‑108 (MQ=255)                                                                                          caccGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGCCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAGTGCTTCTGTCGCTTTATAGCGTTg                                  <  2:71853/139‑1 (MQ=255)                                                                                          caccGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGCCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTg                                  >  1:38495/1‑139 (MQ=255)                                                                                                                          cTCCGGCCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCAccc  <  2:38495/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                      GTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCC  >  NZ_CP009273/1305961‑1306220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑