Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,395,910	2 bp→CC	intergenic (+108/‑96)	purA → / → nsrR	adenylosuccinate synthase/nitric oxide‑sensing transcriptional repressor NsrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,395,910	0	T	C	100.0%	152.7 / NA	43	intergenic (+108/‑97)	purA/nsrR	adenylosuccinate synthase/nitric oxide‑sensing transcriptional repressor NsrR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/16);  total (27/16)
*	NZ_CP009273	4,395,911	0	T	C	100.0%	153.7 / NA	43	intergenic (+109/‑96)	purA/nsrR	adenylosuccinate synthase/nitric oxide‑sensing transcriptional repressor NsrR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/16);  total (27/16)

TGCGCGACCCGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTG  >  NZ_CP009273/4395780‑4396043                                                                                                                                   ||                                                                                                                                     tGCGCGACCCGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTccc                                                                                                                               >  2:363509/1‑139 (MQ=255)   cgcgACCCGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTttccccttcccc                                                                                                                              >  1:368797/1‑138 (MQ=255)   cgcgACCCGTTCGACGCGTAATTCTGGTAAGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGGAAGCCATTACGCTATCCGACACAGAGTTAAATCATCGATTTTCCGCTTcccc                                                                                                                              >  1:368809/1‑138 (MQ=255)                             gTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAg                                                                                                             >  1:190116/1‑129 (MQ=255)                             gTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAg                                                                                                             <  2:190116/129‑1 (MQ=255)                                               ttGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTatcat                                                                                  <  1:133549/138‑1 (MQ=255)                                                        gggCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAAt                                                                        >  2:542063/1‑139 (MQ=255)                                                                       tACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGAt                                                         >  1:465828/1‑139 (MQ=255)                                                                          aTTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTc                                                      >  2:545408/1‑139 (MQ=255)                                                                            tGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTccc                                                    >  1:90905/1‑139 (MQ=255)                                                                            tGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTccc                                                    >  1:389805/1‑139 (MQ=255)                                                                              cTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGaca                                                                                                        <  2:353715/85‑1 (MQ=255)                                                                              cTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGaca                                                                                                        >  1:353715/1‑85 (MQ=255)                                                                              cTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCtt                                                  >  2:323385/1‑139 (MQ=255)                                                                                 tgtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTg                                               >  1:531192/1‑139 (MQ=255)                                                                                     gggTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAgg                                            <  1:337825/138‑1 (MQ=255)                                                                                     gggTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAgg                                            <  1:471106/138‑1 (MQ=255)                                                                                      ggTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTgg                                                                                              >  2:290330/1‑87 (MQ=255)                                                                                      ggTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTgg                                                                                              <  1:290330/87‑1 (MQ=255)                                                                                      ggTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGCATTTTTTCCCGATTTCCCTTTTGAGGtt                                          >  1:440817/1‑139 (MQ=255)                                                                                          aGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtg                                      <  1:307483/139‑1 (MQ=255)                                                                                            ccATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtgtg                                    <  1:545408/139‑1 (MQ=255)                                                                                                tACGCTATCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCt                                                                                            <  2:360275/79‑1 (MQ=255)                                                                                                tACGCTATCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCt                                                                                            >  1:360275/1‑79 (MQ=255)                                                                                                     tATCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAAc                            >  2:211399/1‑138 (MQ=255)                                                                                                       tCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGa                                                          >  1:180053/1‑106 (MQ=255)                                                                                                       tCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGa                                                          <  2:180053/106‑1 (MQ=255)                                                                                                       tCCGACACAGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAg                         >  1:543801/1‑139 (MQ=255)                                                                                                            cacaGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAAc                            <  2:432356/131‑1 (MQ=255)                                                                                                            cacaGTGTTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAAc                            >  1:432356/1‑131 (MQ=255)                                                                                                                 tgtTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgat               >  2:32488/1‑139 (MQ=255)                                                                                                                 tgtTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgat               >  2:579021/1‑139 (MQ=255)                                                                                                                 tgtTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgat               >  2:183695/1‑139 (MQ=255)                                                                                                                 tgtTAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgat               >  1:548036/1‑139 (MQ=255)                                                                                                                   ttAAATCCTCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgatta             <  1:284593/139‑1 (MQ=255)                                                                                                                           tCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGAt                                                         <  1:111932/87‑1 (MQ=255)                                                                                                                           tCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGAt                                                         >  2:111932/1‑87 (MQ=255)                                                                                                                           tCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAAc                            >  1:477446/1‑116 (MQ=255)                                                                                                                           tCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAAc                            <  2:477446/116‑1 (MQ=255)                                                                                                                           tCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggattac     >  2:436477/1‑139 (MQ=255)                                                                                                                           tCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggattac     >  1:232263/1‑139 (MQ=255)                                                                                                                           tCGCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggattac     >  1:71321/1‑139 (MQ=255)                                                                                                                             gCTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacggattac     <  1:363509/137‑1 (MQ=255)                                                                                                                              cTTTTCCCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTg  <  2:389805/139‑1 (MQ=255)                                                                                                                                   ||                                                                                                                                     TGCGCGACCCGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTG  >  NZ_CP009273/4395780‑4396043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑