Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,273,8060GA53.8% 1.1 / 10.3 13Q77Q (CAG→CAA) agaVPTS N‑acetylgalactosamine transporter subunit IIB
Reads supporting (aligned to +/- strand):  ref base G (4/2);  new base A (0/7);  total (4/9)
Fisher's exact test for biased strand distribution p-value = 2.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGG  >  NZ_CP009273/3273732‑3273889
                                                                          |                                                                                   
gCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGtt                                                                      <  1:83893/90‑1 (MQ=255)
    aaGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTa                                                                  >  1:86471/1‑90 (MQ=255)
                         ggACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGa                                             >  2:576655/1‑90 (MQ=255)
                         ggACGCTGAAAAAATTTATCAACAACATTCACCGCGCCGCCGATCGACAAAAAATCCTGCTGGTTTGAAAAACCCCCGCCGATTTCCTGa                                             <  1:372084/90‑1 (MQ=255)
                              cTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTg                                        >  1:426920/1‑90 (MQ=255)
                                         taccGACAACTTCCACCGCCCCGCCGATCGACAAAAAATCCTGCTGGTTTGAAAACCCCCCGCCGATTTCCTGACGCTGGTGAAAGGTgg                             <  1:461374/87‑1 (MQ=255)
                                                      ccccgccCCGCCGACCGAAAAAAAATCCTGCTGGTTTGAAAACCCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAAt                <  2:86471/87‑1 (MQ=255)
                                                      caccgcgCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAAt                <  1:150302/87‑1 (MQ=255)
                                                      cATCGCGCCGCCGATCGACAAAAAATCCTGCTGGTTTGTAAAACCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAAt                <  1:7800/90‑1 (MQ=255)
                                                             ccgccgATCGAAAAAAAATCCTGCTGGTTTGAAAACCCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTa         <  1:199786/90‑1 (MQ=255)
                                                               gccgATCGACAGAAAATCCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAAc       >  1:504461/1‑90 (MQ=255)
                                                                  gATCGAAAAAAAATCCTGCTGGTTTGTAAAACCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGtt    <  1:76510/90‑1 (MQ=255)
                                                                    tCGACAAAAAACCCTGCTGGTTTGTAAAACCCCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTgg  <  1:232698/90‑1 (MQ=255)
                                                                          |                                                                                   
GCAGAAGGGATCGCCGTACGTTTCTGGACGCTGCAAAAAGTTATCGACAACATTCATCGCGCCGCCGATCGACAGAAAATCCTGCTGGTTTGTAAAACACCCGCCGATTTCCTGACGCTGGTGAAAGGTGGCGTTCCGGTGAATCGCATTAACGTTGG  >  NZ_CP009273/3273732‑3273889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑