| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,102,356 | 0 | T | G | 52.6% | 14.5 / 17.8 | 19 | G38G (GGT→GGG) | yiiR | DUF805 domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (3/6); new base G (10/0); total (13/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.10e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.24e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAA > NZ_CP009273/4102269‑4102426 | aTTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGGaa > 1:203331/1‑90 (MQ=255)aTTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGGaa > 1:551347/1‑90 (MQ=255)aTTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTATTCTCACTGGCGGGGaa > 1:247682/1‑90 (MQ=255)aTTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGGGCTTTTCTCACTGGCGGGGaa > 1:371564/1‑90 (MQ=255) gTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGGAAAAAACTACTCCATATTTAGAccc > 2:179281/1‑89 (MQ=255) gATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTtgt < 1:442795/90‑1 (MQ=255) cTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAAACTACTCGATATTCAGACCCCGGCGGTTTTCCTTGTGtgcttt > 1:561256/1‑89 (MQ=255) gtgGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCgcgg > 1:178831/1‑68 (MQ=255) gtgGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCgcgg < 2:178831/68‑1 (MQ=255) gtgGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGGAAGAAATTACTCGATATTTAGACCGCGGGGGTTTTCCTTTTGtggtttct > 1:555007/1‑90 (MQ=255) gtgGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGGAAGAAACTACTCGATAATCAAACCCCGGCGGTTTTGCTTGTGtggttggt > 2:56873/1‑88 (MQ=255) tCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGGAAGAATCTACTCGATATTCAGACCGCGGCGGTTTTCCTTGTGTGGTTTTTCTggg > 1:402034/1‑89 (MQ=255) tCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGGAAGAAACTACTCGATTTTCAGACCGCGGGGGTTTTCCTTTTGTGGTTGCTCTTGc > 1:391841/1‑90 (MQ=255) ggCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCCCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACa > 1:179838/1‑90 (MQ=255) cTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATACTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCa < 1:56873/90‑1 (MQ=255) tGCTTTTCTCACTGGCGGGTAAGAGTCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTaa < 2:381156/90‑1 (MQ=255) tGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTaa < 1:443645/90‑1 (MQ=255) tGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTaa < 1:359324/90‑1 (MQ=255) tGCTTTTCTCACTGGCGGGGAAGAAAATACTCGATATTCAAACCCCGGCGTGTTTCCTTGTGTGGTTGCTCTGGGCCACAGCGGGAGGaa > 2:275388/1‑90 (MQ=255) | ATTTAAAGGGCGTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAA > NZ_CP009273/4102269‑4102426 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |