| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 228,175 | 0 | A | G | 53.3% | 8.2 / 14.7 | 16 | G189G (GGA→GGG) | yafD | endonuclease/exonuclease/phosphatase family protein |
| Reads supporting (aligned to +/- strand): ref base A (2/5); new base G (8/0); total (11/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAG‑AAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGT > NZ_CP009273/228094‑228249 | gggCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCaa < 1:321209/90‑1 (MQ=255) gTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGTTTTTATGCCTggggg > 1:273064/1‑87 (MQ=255) gTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGc < 1:569029/90‑1 (MQ=255) taGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAg < 1:52895/90‑1 (MQ=255) tAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAG‑AAg < 2:392159/90‑1 (MQ=255) aaGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGTTTTTATATCTGGGGCCGGag‑gagg > 2:109610/1‑90 (MQ=255) aaGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAAGTTTTCATCCCCGGGGCCGGAG‑AAgg > 1:193551/1‑90 (MQ=255) aGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGATTTTAATGCCCGGGGGCGTAG‑AAGGa > 1:246898/1‑90 (MQ=255) gTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGTTTTAATGCCTGGGGGCGGAG‑AAGGGTGaa > 1:245267/1‑90 (MQ=255) tACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGGTTTAAATCCTCGGGGCGGAG‑AAGGAGGaaag > 2:559393/1‑88 (MQ=255) aTTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTTAATTCCTGGGGCCGCAG‑AAGGAAGAAAGCGTTatat > 1:316549/1‑90 (MQ=255) gATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGAGTTTAATGCCTGGGGGCGTAG‑AAGGAGGAACGCGTTTTATTGCttt > 1:261015/1‑90 (MQ=255) aGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAG‑AAGGATGAACGCGTTATATCGCTTTgcgc < 1:211808/90‑1 (MQ=255) accacAGCGGCCCGGTCATTATGGCGGGGGATTTTAATTCCCGGAGGCGGAGAAAGGAGGAA‑GCGGTTTATCGCTTTGGGCGGGGAAAGt > 1:442031/1‑90 (MQ=255) ccGGTCATTATGGCGGGGGATTTTTATTCCTGGGGCCGTAG‑AAGGATGAACGCGGTTTATTGCTTTTTGGGGGGAATTTTGCTGCGcccg > 1:309736/1‑88 (MQ=255) ggTCATTATGGCGGGCGAGTTCAATAACCGGGGCCGCAA‑AAAGGCGAAAGCGGTTTATCTGTTTGTGCGGGGAATGTCCCTGCCCCCggg > 1:416738/1‑89 (MQ=255) | GGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAG‑AAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGT > NZ_CP009273/228094‑228249 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |