| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,227,178 | 0 | T | C | 58.3% | 5.7 / 11.3 | 12 | D118D (GAT→GAC) | yjbG | capsule biosynthesis GfcC family protein |
| Reads supporting (aligned to +/- strand): ref base T (5/0); new base C (0/7); total (5/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCCAGCGCCGACGATGCCGCTGCGATTAACGCCTTACGCCAGCAAATTCAGGCGTTGAAAGTGACGGGCAGGCAAAAAATCAATCTTGATCCCGATATCGTCCGCGTTGCCGAACGCGGTAACCCGCCGTTGCAGGGCAACTACACGCTGTGGGTCGGACCACCGCCGTCCAC > NZ_CP009273/4227089‑4227261 | tCCAGCGCCGACGATGCCGCTGCGATTAACGCCTTACGCCAGCAAATTCAGGCGTTGAAAGTGACGGGCAGGCAAAAAATCAATCTTGAt > 2:171904/1‑90 (MQ=255) ccGACGATGCCGCTGCGATTAACGCCTTACGCCAGCAAATTCAGGCGTTGAAAGTGACGGGCAGGCAAAAAATCAATCTTGATCCCGata > 2:254060/1‑90 (MQ=255) tACGCCAGCAAATTCAGGCGTTGAAAGTGACGGGCAGGCAAAAAATCAATCTTGATCCCGATATCGTCCGCGTTGCCGAACGCGGTAAcc > 2:556261/1‑90 (MQ=255) gCAAATTCAGGCGTTGAAAGTGACGGGCAGGCAAAAAATCAATCTTGATCCCGATATCGTCCGCGTTGCCGAACGCGGTAACCCGCCGtt > 1:327872/1‑90 (MQ=255) cAGGCGTTAAAAGTGAGGGGCAGGAAAAAAATCAATCTTGACCCCGATACCGCCCGCGTTGCCGAACGCGGTACCCCGCCGTTGCAGGGc < 2:167760/90‑1 (MQ=255) gTTGAAAGTGACGGGCAGGCAAAAAATCAATCTTGATCCCGATATCGTCCGCGTTGCCGAACGCGGTAACCCGCCGTTGCAGGGCAACTa > 1:555056/1‑90 (MQ=255) aaaaGTGACGGGCAGGAAAAAAATCAATCTTGACCCCGATATCGCCCGCGTTGCCGAACGCGGTAACCCGCCGTTGCAGGGCAACTacac < 1:383495/89‑1 (MQ=255) aaGTGACGGGCAGGAAAAAAATCAATCTTGACCCCGATATCGTCCGCGTTGCCGAACGGGGTACCCCGCCGTTGCAGGGCAACTACACGc < 1:556261/90‑1 (MQ=255) gTGACGGGCAGCCAAAAAATCAATCTTGACCCCGATACCGCCCGCGTTGCCAAACGCGGTAACCCGCCGTTGCAGGGCAACTACACGCtg < 1:130154/90‑1 (MQ=255) ggcaggaaAAAAATCAATCTTGACCCCGATATCGTCCGCGTTGCCGAACGCGGTACCCCGCCGTTGCAGGGCAACTACACGCTGTGGGTc < 1:139698/90‑1 (MQ=255) ggcaAAAAATCAATCTTGATCCCGATATCGTCCGCGTTGCCGAACGCGGTAACCCGCCGTTGCAGGGCAACTACACGCTGTGGGTCGGac > 1:88762/1‑90 (MQ=255) aaaCCAATCTTGACCCCGATATCGCCCGCGTTGCCGAACGGGGTACCCCGCCGTTGCAGGGCAACTACACGCTGTGGGTCGGACCAccgc < 1:482102/90‑1 (MQ=255) tcTTGACCCCGAAATCGCCCGGGTGGCCGAACGGGGTACCCCGCCGTTGCAGGGCAACTACACGCTGTGGGTCGGACCACCGCCGTCCAc < 2:77115/90‑1 (MQ=255) | TCCAGCGCCGACGATGCCGCTGCGATTAACGCCTTACGCCAGCAAATTCAGGCGTTGAAAGTGACGGGCAGGCAAAAAATCAATCTTGATCCCGATATCGTCCGCGTTGCCGAACGCGGTAACCCGCCGTTGCAGGGCAACTACACGCTGTGGGTCGGACCACCGCCGTCCAC > NZ_CP009273/4227089‑4227261 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |