| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 297,072 | 0 | A | C | 60.0% | 7.4 / 12.3 | 15 | V176G (GTG→GGG) | paoB | aldehyde oxidoreductase FAD‑binding subunit PaoB |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/9); total (5/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTTCAATGTGCGGCGTTTTTCCCGGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCC > NZ_CP009273/296985‑297145 | gTTTCAATGTGCGGCGTTTTTCCCGGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTccacc > 2:88018/1‑90 (MQ=255) aaTGTGCGGCGTTTTTCCCGGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGc > 2:577227/1‑90 (MQ=255) tgtgCGGCGTTTTTCCCGGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCAt < 1:535964/90‑1 (MQ=255) tttttCCCGGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAAcc > 1:573987/1‑90 (MQ=255) gAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGa > 1:295322/1‑90 (MQ=255) aaaTCAGCCAGTGTGATCCTGCGAGTCTTCCCCCCCGGCGTGATGGTTTCCCCCCCCGCATCCAGCACCCGCTTTGCGACCGCCATATCg < 1:398076/90‑1 (MQ=255) ccAGCCGGTGTGATACTGCGTTTTTTCCCCCCGGGGGTGTTGTTTTCCCCCCCCGCATCCAGCACCCGCATTGCGACCGCCATATCGCTc < 2:295322/89‑1 (MQ=255) cAGCCAGTGTGATCCTGCGATTCTTTCCCTCCGGCGGGATGTTTTCCCCCACCGCATCCACCAACCGCTTTGCGACCGCCATATCGCTCg < 1:369810/90‑1 (MQ=255) gtgtGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATggg > 1:563489/1‑90 (MQ=255) gAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAgg > 2:109069/1‑90 (MQ=255) tCTTCCCCCCCGGCGTGATGGTTCCCCCCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCtt < 1:88018/90‑1 (MQ=255) cccccccGGGGTGATGTTTCCCCCCCCCCCCCCCCGCACCCCCTTGGCCCCCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCt < 2:307556/89‑1 (MQ=255) cccccGGCGTGATGTTTCCCCCCCCCGCACCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTa < 1:383228/87‑1 (MQ=255) cccccGGCGTGATGGTTTCCCCCCCCGCACCCAGCACCCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTa < 1:389180/87‑1 (MQ=255) ccTCCGGCGTGATGGTTCCCCCCCCCGCACCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTa < 1:253605/90‑1 (MQ=255) cGGCGTGTTGTTTCCCCCCCCCGCACCCACCACCCGCTTTGCGCCCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGcc < 2:563489/90‑1 (MQ=255) | GTTTCAATGTGCGGCGTTTTTCCCGGAGGGTGATAAAAATCAGCCAGTGTGATACTGCGAGTCTTTCCCTCCGGCGTGATGGTTTCCACCACCGCATCCAGCAACCGCATTGCGACCGCCATATCGCTCGGATGGGTGGCAATGCAGGCTTCGCTTACGCC > NZ_CP009273/296985‑297145 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |