| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 333,785 | 0 | G | T | 62.5% | ‑0.9 / 13.9 | 16 | V433L (GTG→TTG) | fdrA | acyl‑CoA synthetase FdrA |
| Reads supporting (aligned to +/- strand): ref base G (3/3); new base T (10/0); total (13/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.57e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.57e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC > NZ_CP009273/333703‑333865 | gatgatTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTTTGGTCgg > 1:489740/1‑90 (MQ=255) gACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGGGGTCGTGATGGGGTTTTTGCTCGGGTTTggg > 2:223970/1‑89 (MQ=255) gACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGGGATCGGGATGGGTTTTTTGCTCTGGTTTggg > 1:550695/1‑89 (MQ=255) ccaccaACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGAGATCGGGATGGGTTTTTTGCTCGGATTTTGGTCGc > 1:411634/1‑90 (MQ=255) cAGTCGCTTCATGGAAGAGGCGGGCGTGCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGAGCGCATGAAGATCCGGt < 2:92057/90‑1 (MQ=255) gTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATTGTGATGGATTTTTTTCTCGGGTTTGGATTGCATGAAGATTCGGGCg > 1:604477/1‑90 (MQ=255) cGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTTTGCTc > 1:194625/1‑60 (MQ=255) cGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTc < 2:194625/60‑1 (MQ=255) ttGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTTTGCTCGGGTTTGGATCGCATGAAGATCCGGGCGGCTcc > 1:19505/1‑90 (MQ=255) gcgcgcGATCCAGAAGTGGCGGTGGTCGTGGTGGATTTTGTGGTCGGGTTTTGGTCGCATGAAGAACCGGGCGGCCCCCCCCTCGAGACg > 2:98479/1‑90 (MQ=255) gcgcgATCCAGAAGTGGCGGTGGTCGTGATGGGTTTTTTGCTCGGATTTGGATCGCATGAAAATCCGGGCGGCTCCACCATCCAGACGAt > 1:459106/1‑90 (MQ=255) ggCGGTGATCGTGGTGGATTTTGTGCTCGGATTTTGATCGCCTGAAAATCCGGGCGGCTCCACCATCGGGACGATCAAAGaaaggaaagc > 2:387315/1‑90 (MQ=255) ggCGGTGATCGTGATGGATTTTTTGCTCGGGTTTTGATCGCATGAAGATCCGGTCGGCTCCCCCCTCGAGACGATCAAAAaagcgaaaaa > 2:250650/1‑88 (MQ=255) ggCGGTGATCGTGATGGATTTTTTGCTCGGGTTTGGATCGCATGAAGATCCGGGCGGCTCCACCATCGAGACGATCAAAAaagcgaaaac > 2:210908/1‑88 (MQ=255) cGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATcgccgc < 2:158234/90‑1 (MQ=255) aTGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGcc > 1:182524/1‑90 (MQ=255) | GATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCC > NZ_CP009273/333703‑333865 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |