Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273585,3930CG58.3% 5.1 / 13.7 12A340P (GCC→CCC) nfrAbacteriophage adsorption protein NfrA
Reads supporting (aligned to +/- strand):  ref base C (1/4);  new base G (7/0);  total (8/4)
Fisher's exact test for biased strand distribution p-value = 1.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAA  >  NZ_CP009273/585324‑585480
                                                                     |                                                                                       
cAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGAGGAGGTTTTTCGCTGcgg                                                                     >  1:49959/1‑89 (MQ=255)
      tGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGGTTTTTCGCTTCGGCGTACt                                                               >  1:86264/1‑90 (MQ=255)
          aggggTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGAcc                                                           <  1:318344/88‑1 (MQ=255)
          aCGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGAcc                                                           <  2:235362/90‑1 (MQ=255)
          aCGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGAcc                                                           <  2:485216/90‑1 (MQ=255)
                             gCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGt                                        <  2:508432/90‑1 (MQ=255)
                                       cctcAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGTTTTTTCGCTGCGTCGTACTGACCTTCTTTTTAAAGCACCCGGAGCGTCgcgc                              >  2:224219/1‑90 (MQ=255)
                                       cctcAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGGTTTTTCGCTGCGGCGTACTGACCTTCTTTTTACAGCACCCGGAGGGTCgcgc                              >  2:463788/1‑90 (MQ=255)
                                             gCATTTCATTGGCGGGGGGGGGGGGGAGCAGGTTTTTCGCTGCGTCGTACTGACCTTCTTTTTACAGCACCGGTAGCGGCGCGCccacaa                        >  1:32914/1‑90 (MQ=255)
                                               aTTTCATTGGCGGGGGGGGGGGGGGGCAGGTTTTTCGCTGCGGCGTACTTACCTTCTTTTTAAAACACCGGGAGCGGCGCGCCCacaaca                      >  2:76580/1‑90 (MQ=255)
                                               aTTTCATTGGCGGGGGGGGGGGGGAGCAGGTTTTTCGCTGCGGCGTACTGACCTTCTTTTTAAAGCACCGGGAGCGGCGCGCCCacaaca                      >  2:76579/1‑90 (MQ=255)
                                                                   ggCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCaaa  >  1:190036/1‑90 (MQ=255)
                                                                     |                                                                                       
CAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGGCAAA  >  NZ_CP009273/585324‑585480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑