| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 702,597 | 0 | T | G | 56.2% | 10.8 / 13.9 | 16 | V350G (GTG→GGG) | glnS | glutamine‑‑tRNA ligase |
| Reads supporting (aligned to +/- strand): ref base T (4/3); new base G (9/0); total (13/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.25e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGC > NZ_CP009273/702515‑702679 | aTTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATTGATCCGGGGAAACTg > 1:511508/1‑90 (MQ=255)aTTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATTGATCCGGGGAAAATg > 1:507956/1‑90 (MQ=255)aTTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGGGaaaacg > 1:202971/1‑87 (MQ=255)aTTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGGTATCGATCCCGGGAAACTg > 2:107720/1‑90 (MQ=255)aTTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGC‑GTTATCGATCCGGTGAAACTgg > 2:544937/1‑90 (MQ=255) ggCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGGGAAACTGGTTATCGa > 1:166520/1‑90 (MQ=255) ggCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGGTTTTCATCCTGGGAAAGAGGTTTTCgg > 2:394821/1‑89 (MQ=255) tGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGggcgaa < 2:128317/90‑1 (MQ=255) gTGAAGATCTCAACGAAAATGCGCCGCGCGCGATGGCGGTTATCGATCCGGGGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGaaa > 2:396775/1‑90 (MQ=255) gAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGaaa < 1:338993/67‑1 (MQ=255) gAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGaaa > 2:338993/1‑67 (MQ=255) tctcAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTAc < 1:27080/90‑1 (MQ=255) tctcAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCCGGGGAAATGGTTATTGAAAAACATCAGGGGGAAGGGGAAAAGGGTAc > 2:501259/1‑90 (MQ=255) gccgcGCGCAATGGCGGTTATCGATCCGGGGAAAATGGGTATCGAAAACTATCAGGGGGGAGGCGAAAAGGGTACCATGCCCAAACCTcc > 2:221291/1‑90 (MQ=255) gcgcgcAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGaa > 1:571522/1‑90 (MQ=255) gATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGc > 1:158915/1‑90 (MQ=255) | ATTGAGATGGCGTCGCTGGAATCCTGCATCCGTGAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGC > NZ_CP009273/702515‑702679 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |