| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,356,152 | 0 | C | A | 40.9% | 28.5 / 10.2 | 22 | A321S (GCC→TCC) | yfaY | nicotinamide mononucleotide deamidase‑related protein YfaY |
| Reads supporting (aligned to +/- strand): ref base C (10/3); new base A (0/9); total (10/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCG‑AAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCCAGCAA > NZ_CP009273/2356073‑2356239 | gCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCG‑AAACAGCCAGTGCCAGCCCGGCAAAATGGTTg > 2:406798/1‑90 (MQ=255)gCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCG‑AAACAGCCAGTGCCAGCCCGGCAAAATGGTTg > 2:157604/1‑90 (MQ=255) aaGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCG‑AAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGccc > 1:366370/1‑90 (MQ=255) cccGTCGGGGGGGGCTGGCGAAAAGTTGAGATCCTCTTTCCCAAAACCAA‑AACCAGCCAGTGCCAGCCCGGCAAAATGGTTGGCccgccg < 2:212839/89‑1 (MQ=255) ccGTCGGGGGTGGCTAGCGAAAAGTGGAGATGCTCTTTCTCAAACCCAA‑AAACAGCCAGTGCCACCCCGGAAAAATGGTTGGCCCGCCGt < 2:290216/90‑1 (MQ=255) cGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCG‑AAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGtt > 1:212839/1‑90 (MQ=255) gTCTGGCGTGGCTAGCGAAAAGTTGAGATGCTCGTTCTCAAAACCCG‑AAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTc < 1:683200/90‑1 (MQ=255) gTCGGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCG‑AAACAGCCAGTGCCAGCCCGGAAAAATGGTTGGCCCGCCGTTc < 1:34864/90‑1 (MQ=255) gTCGGGCGTGGCTAGCGAAAAGTTGAAAACCTC‑TTCCCCAAACCCGAAAACAGCCAGTGCCAGCCCGGAAAAATGGTGGGCCCGCCGTTc < 2:617647/90‑1 (MQ=255) gggggTGGCGGGCGAAAAGTGGAGTTTCTCGTTCCCAAACCCCA‑AACCAGCAAGTGCCAGCCGGAAAAAATGGTTGCCCCGCCGTTCTGt < 2:649502/86‑1 (MQ=255) gCTCGTTCTCGAAACCCG‑AAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAggg > 1:467245/1‑90 (MQ=255) tCGTTCTCGAAACCCG‑AAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGtt > 1:546312/1‑90 (MQ=255) cccA‑AAACACCCGGTGCCACCCCGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGaa < 1:350144/90‑1 (MQ=255) aCAGCCGGGGCCAGCCCGGAAAAAGGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAcc < 2:93260/90‑1 (MQ=255) ccAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACtt > 1:144576/1‑90 (MQ=255) ccAGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACg < 1:108444/90‑1 (MQ=255) aGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGcc < 1:105318/90‑1 (MQ=255) gCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCCa > 2:8263/1‑90 (MQ=255) cGGAAAAAGGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCCAGCa < 1:154974/90‑1 (MQ=255) ggCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGa < 1:62078/66‑1 (MQ=255) ggCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGa > 2:62078/1‑66 (MQ=255) ggCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCCAGCaa > 2:593706/1‑90 (MQ=255) | GCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCG‑AAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCCAGCAA > NZ_CP009273/2356073‑2356239 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |