| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,738,694 | 0 | T | C | 57.1% | 0.8 / 10.8 | 14 | P177P (CCT→CCC) | yiaN | 2,3‑diketo‑L‑gulonate transporter large permease YiaN |
| Reads supporting (aligned to +/- strand): ref base T (4/2); new base C (8/0); total (12/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGC > NZ_CP009273/3738615‑3738782 | ccccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCGTTTCCCCCGGCctgatga > 2:662875/1‑90 (MQ=255) ttCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCCGGGATTATGATggg > 2:4069/1‑90 (MQ=255) cATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGgcgc < 2:371947/90‑1 (MQ=255) gTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGATTTCCCCCGGCATGATGATGGGGGCAACGCt > 2:21068/1‑90 (MQ=255) gTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCCGGCATGATGATTGGGGCAACGCt > 2:87775/1‑90 (MQ=255) ttCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTTCCCCTGGCATGATGATGGGGGCAACGCTGATGCTTTcc > 1:541937/1‑90 (MQ=255) ttCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCCGGCATGATGATGGGCGCAACGCTGATGCTTTcc > 1:574976/1‑90 (MQ=255) cAGTAAGCTGTTTATGGCGGGCATTGCCCCCGGCATGATGATGGGGGCAACGCTGATGCTTTCCTGGTGGTGGCAGGGCCGGCGCCTTaa > 2:484858/1‑90 (MQ=255) aaGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTa > 1:164472/1‑90 (MQ=255) gTTTATGGCGGGCATTGCCCCCGGCATGATGATGGGGGCAACGCTGATGCTTTCCTGGGGGGGGGAGGCCAGTCGCCTTTATTTAccccc > 1:630090/1‑89 (MQ=255) ggCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCtggtg < 1:56423/50‑1 (MQ=255) ggCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCtggtg > 2:56423/1‑50 (MQ=255) ggCATTGCCCCCGGCATGATGATGGGGGCAACGCTGATGCTTTCCTGGGGGGGGGAGGGCCGGCGCCTTAATTTTACCCCCCCGCaaaaa > 2:106795/1‑90 (MQ=255) cTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGc > 1:744/1‑90 (MQ=255) | CCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCATTGCCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTACCCCGCCAGCAAAAAGCAACGATGC > NZ_CP009273/3738615‑3738782 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |