| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,776,279 | 0 | T | A | 40.5% | 27.0 / 11.4 | 38 | D248V (GAT→GTT) | gpsA | NAD(P)H‑dependent glycerol‑3‑phosphate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (11/11); new base A (15/0); total (26/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.04e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTG > NZ_CP009273/3776193‑3776365 | gcgcgcTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGGTGTCGGGACAGGGAAGGACCAAATCGc > 1:442743/1‑90 (MQ=255) gcgcTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTCCCGACTGGTTTTCGGGACCGGTAAACACCAAAACGCCa > 2:86483/1‑90 (MQ=255) tCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTCCCCCCCTGGTGTGGGGAAAGGTAAACACCAAAACGCCAAACccccccccg > 2:631402/1‑87 (MQ=255) tCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATg < 1:405282/90‑1 (MQ=255) tCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATg < 1:494639/90‑1 (MQ=255) accgtgccctgcccGAGCACCATGCCAAAACGGCGGTTACGCGATTGGTTGTCGGTAGAGGTAAGCCCAAAACCGCCAAGCCCCGCCATg < 2:606612/86‑1 (MQ=255) ccATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACCTGGTGTCGGGACAGGGAAACACCCAAACGCCAAGCcccccccccc > 2:405282/1‑86 (MQ=255) aTGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGGCTGGTTGTCGGGACAGGTAAGCACCAAAACGCCAAGCCCCCCCCTGccc > 1:412177/1‑90 (MQ=255) aTGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGGCTGGTTGTCGGGACAGGGAAGCACCAGATCGCCAAGCCCCCCCCTGccc > 2:691826/1‑90 (MQ=255) aTGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATg < 2:249020/87‑1 (MQ=255) aTGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGGACAGGTAAGCACCAGATCGCCAAGCCCCGCCATg > 1:249020/1‑87 (MQ=255) aTGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGGACAGGGAAACACCAAAACGCCAAACCCCCCCCTGccc > 1:540144/1‑90 (MQ=255) gCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGGAAGCACCAGATCGCCAAGCCCCGCCATGCCCAt > 1:579354/1‑90 (MQ=255) gACCGAGCATCATGCCAAAACGGCGGTTACGCGGCTGGGTGTCGGTACAGGGAAGCACCAGAACGCCAAACCCCCCCCTGCCCCCAAAgg > 2:128993/1‑90 (MQ=255) gACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGGACAGGGAAGCACCAGAACGCCAAGCCCCCCCCTGCCCCTAAAgg > 2:111218/1‑90 (MQ=255) gACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGGTGTCGGGACAGGGAAGCACCAAAACGCCCAACCCCCCCCTGCCCCTaaaag > 1:447700/1‑88 (MQ=255) gACCGAGCATCATGCCAAAACGGCGGGTACGCGACTGGTTGTCGGGACAGGGAAGCACCAGAACGCCCAACCCCCCCACGCCCATaaaag > 2:494639/1‑88 (MQ=255) gAGCATCATGCCAAAACGGCGGTTACGCGGCTGGTTGTCGGGACAGGTAAGCACCAAAACGCCAAGCCCCCCCCTGCCCCTAAAAGGGGc > 2:647029/1‑90 (MQ=255) aGCATCATCCCAAAACGGCGTTTCCGCGACTGGTTGTCGGTACGGGTAAGCCCCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCa < 2:442743/90‑1 (MQ=255) gCATCATGCCAAAACGGCGGTTACGCGGCTGGTTGTCGGCGCAGGGAAACACCAAAAACCCCACCCCCCCCCTGCCCCTAAAAGGGGCAg > 2:111194/1‑90 (MQ=255) catcatGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAgg < 1:691826/90‑1 (MQ=255) atGCCAAAACGGCGGTTACGCGACTGGTTGTCGGGACAGGGAAACACCCAAACGCCAAGCCCCCCCCTGCCCCTAAAGGGGGGAGGGGCg > 2:343009/1‑90 (MQ=255) atGCCAAAACGGCGGTTACGCGACTGGGTGTCGGGACAGGGAAGCACCAAAACGCCAAACCCCCCCCTCCCCCTAAAAGGGGGAGGGGCg > 2:397727/1‑90 (MQ=255) tGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCCCCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCgg < 1:343009/90‑1 (MQ=255) tGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCgg < 1:86483/90‑1 (MQ=255) aaaCGGCGGTTACGCGACTGGGTGGCGGGACCGGGAAGCACCAAAACCCCAACCCCCGCCATGCCCCAAAAGGAGGCAGGGGCGGCAccc > 1:565400/1‑90 (MQ=255) gcggTTCCGCGCCTGGTTGTGGGTACGGGAAAGCCCCAGATCGCCAACCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAgcgc < 2:540144/90‑1 (MQ=255) gcggTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAgcgc < 1:128993/90‑1 (MQ=255) gcggTTACGCGACTGGGTGTTGGTGCAGGGAATCACCAAAACGCCCACCCCCCCCCTGCCCCCAAAGGGGGGGGCGGCGGGACCCatacg > 2:409260/1‑86 (MQ=255) tGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGAcgc > 2:162440/1‑90 (MQ=255) cAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTcagcc > 2:247155/1‑90 (MQ=255) ggTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCTGCACCCAGCGCCGCACCAAGACGCGACATTTcagccag > 1:512570/1‑90 (MQ=255) aaGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGccc > 2:642800/1‑90 (MQ=255) gCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCAc > 1:431581/1‑90 (MQ=255) cACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACg < 1:542767/90‑1 (MQ=255) caaGATCCCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACggg < 2:107508/88‑1 (MQ=255) aGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCGCGGGTg > 1:44441/1‑90 (MQ=255) aGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTg > 1:682754/1‑90 (MQ=255) | GCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTG > NZ_CP009273/3776193‑3776365 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |