| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 68,443 | 0 | T | G | 60.0% | 8.9 / 11.9 | 15 | G202G (GGT→GGG) | yabI | DedA family protein |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (9/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.38e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTG > NZ_CP009273/68356‑68527 | gagCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGGaa > 1:265684/1‑90 (MQ=255) gTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCCGGGGTAAAgggg > 2:567385/1‑87 (MQ=255) gAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGGGCCGCGGGAAAGCGGCt > 2:671656/1‑90 (MQ=255) tttAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGGAAAGCGACTGAc > 1:535201/1‑90 (MQ=255) aaTGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTTTGGGGCCGGGGGAAAGGGACTGACCggt > 2:48164/1‑88 (MQ=255) aTGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGttt < 1:671656/90‑1 (MQ=255) gTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAg < 1:344324/90‑1 (MQ=255) ttGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATTGGGCAGCGGGAAAACGACCGACCGgtttggt > 1:702621/1‑86 (MQ=255) ttGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCCGCGGGAAAAAGACCGACCGTTTGAGt > 1:314850/1‑90 (MQ=255) tgGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCattatt < 2:280041/90‑1 (MQ=255) cAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTTTGGGGCAGCGGGAAAGCGACTGACCGTTTTTGTTATTATTTTTccc > 1:444220/1‑90 (MQ=255) gtTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGGGCAGCGGGAAAACGACTGACCGTTTTGGGCATTTTTTTTCCCCCGGGCg > 1:485508/1‑90 (MQ=255) tggctggcTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGAc < 2:560116/90‑1 (MQ=255) ggctgTGCTGGCGGTTATGGCGCAGCGGGAAAGCGACTGGCCGTTTGGGTCCTTATTTTTCCCGCGGGCGTTTGTTGTGGGTGGAGCCgg > 2:405461/1‑89 (MQ=255) aGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCgtggtg < 1:603992/90‑1 (MQ=255) | GAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTG > NZ_CP009273/68356‑68527 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |