| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,927,661 | 0 | A | T | 52.4% | 7.3 / 29.6 | 21 | S401T (TCA→ACA) | edd | phosphogluconate dehydratase |
| Reads supporting (aligned to +/- strand): ref base A (9/1); new base T (0/11); total (9/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.40e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.06e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTG‑TTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTG > NZ_CP009273/1927581‑1927741 | cAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTG‑TTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGc < 2:154327/90‑1 (MQ=255) ccGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTG‑TTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTcc > 2:476149/1‑90 (MQ=255) ccGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTG‑TTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTcc > 1:244728/1‑90 (MQ=255) gCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTG‑TTCGAAGGAAGCGAGCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCg > 1:389565/1‑90 (MQ=255) ttAACACTTTTGTCCCACCATGATGAGAGAAAGGTTG‑TTAGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGcc > 1:531382/1‑90 (MQ=255) tAACACTTTTGTCCCACCATGATGAGAGAAAGGTTG‑TTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGcca > 2:316954/1‑90 (MQ=255) tttGTCCCACCATGATGAGAGAAAGGTTG‑TTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGccagtccctg > 1:398410/1‑87 (MQ=255) gagagaAGGGTTG‑TTCGAGGGAGGCGATCACTTTGCTGTCGAGTGTTTTTCCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGcc < 2:259349/90‑1 (MQ=255) gaaaaaGTTTT‑TTCGAAGGAAGCGATCACTTTGCTGTCGAGTGTTTTTCCCCCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCAt < 2:79106/87‑1 (MQ=255) gaaaaaGTTTG‑TTCGAAGAACGCAACCACTTTGCTGTGGATTTTTTTTCCCCCCCCTCCCCGCCAGTCCAGTTCACCATTATTCAGCCAt < 2:601914/87‑1 (MQ=255) gaaaaaGTTTG‑TCCGAAGGAAGCGATCACTTTGTTGTGGAGTGTTTTTCCCCCCCCTCCCCGCCAGTCCAGTTCACCATTATTCAGCCAt < 1:310668/87‑1 (MQ=255) gaaaaGGTTTTTTCGAAGGAAGCGATCACTTTGCTGTCGATTTTTTTTTCCCCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCAt < 2:244728/88‑1 (MQ=255) ggTTG‑TTCAAGGGAAGCGATCCCTTTGCTGTCGAGTGTTTTTCCCCCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCa < 2:115965/90‑1 (MQ=255) tttg‑ttCAAAGAAAGCGATCCTATTGCTGTCGAGTGTTTTTTCCCCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCaa < 1:558223/89‑1 (MQ=255) g‑ttCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAggg > 1:269873/1‑90 (MQ=255) ttCGAAGGAAGCAACCACTTTGCTGTCGGGTGTTTTTCCCCCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGt < 2:269873/90‑1 (MQ=255) gCGATCCCTTTGTTGTGGAGTTTTTTTTCCCCCCCTCCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagac < 1:476149/90‑1 (MQ=255) ctttgttgTCGCGTTTTTTTCCCGCCCCTCCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCaa < 1:520131/88‑1 (MQ=255) tGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTCCAAGGGTATAACGAGACAGACCAAAACCTg > 1:34491/1‑90 (MQ=255) gAGTGTTTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagac < 1:81954/73‑1 (MQ=255) gAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagac > 2:81954/1‑73 (MQ=255) | CAGGTTACCGCTTAACACTTTTGTCCCACCATGATGAGAGAAAGGTTG‑TTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGACAGACCAAAACCTG > NZ_CP009273/1927581‑1927741 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |