| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,141,214 | 0 | T | G | 57.1% | 10.5 / 14.5 | 14 | G20G (GGT→GGG) | yegD | molecular chaperone |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (8/0); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATGGC > NZ_CP009273/2141138‑2141302 | ggCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTGATGCGTGGCGGGAAAACGCAttttt > 2:524696/1‑88 (MQ=255) tCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGGGGGGAAACCCCCTTTTGTAAAAAAGGaaaaag > 1:555240/1‑88 (MQ=255) tATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCAc < 2:678979/90‑1 (MQ=255) gTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACgctgc < 2:488868/90‑1 (MQ=255) ttttGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGGGACGGGAAACCCCATTTGCTTAAAAAGGAAAAAGACAGCCCgccgct > 1:407772/1‑90 (MQ=255) tttGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGctgctg < 1:472751/90‑1 (MQ=255) aaCTGTTCAGTGGCGGTCATGCGTGACGGGAAACCCCCTTTTCTAAAAAAGGAAAAAGACAGCACGCTGCTGCCTTCCATGCTTTtcgcg > 2:191191/1‑90 (MQ=255) cTGTTCAGTGGCGGTCATGCGTGACGGGAAAACGCCTTTTCTAAAAAAGGGAAAAGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGcc > 2:188495/1‑90 (MQ=255) cTGTTCAGTGGCGGGCATGCGTGGCGGGAAAACCCACTTTCTAAAAAAGGAAAACGACAGCACGCCGCTGCCCTCCATTCTTTGCGCGcc > 2:29323/1‑90 (MQ=255) tGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCa < 2:590664/90‑1 (MQ=255) gTGGCGGGCATGCGGGACGGGAAAACGCATTTTCTAAAAATGGAAAAAGACAGCACGCTGCTGCCTTCAATGCTTTTCGCGCCAAcgcgg > 2:319069/1‑89 (MQ=255) gCGGTCATGCGTGACGGGAAAACGCATTTTCTAAAAAAAGAAAAAGACAGCACGCTGCTGCCTTCAAAGCTTTTCGCGCCCACGCGGGaa > 2:206823/1‑90 (MQ=255) aTGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTa < 2:373141/90‑1 (MQ=255) gTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATGGc < 2:391818/90‑1 (MQ=255) | GGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGACGGTAAACCGCATTTGCTAAAAATGGAAAACGACAGCACGCTGCTGCCTTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCGAATGGC > NZ_CP009273/2141138‑2141302 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |