| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,354,816 | 0 | A | C | 52.9% | 9.4 / 17.6 | 17 | G26G (GGT→GGG) | rhmD | L‑rhamnonate dehydratase |
| Reads supporting (aligned to +/- strand): ref base A (5/3); new base C (0/9); total (5/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.05e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATG > NZ_CP009273/2354740‑2354897 | cGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAgcgc < 2:83509/90‑1 (MQ=255) gcgGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTc > 1:181938/1‑90 (MQ=255) cATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCAcc < 1:287843/90‑1 (MQ=255) cATCGGGGTGGAAATATGACCTTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCCCCGCCGCCAGCGCCTTTTTCTGCTGTCGCAcc < 1:580949/90‑1 (MQ=255) agcGGGGTGGAAATATGTTCGTCTTCCCAGTGATTCGCCCCCTGGTCGTGATAATCCCCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCg < 2:54217/88‑1 (MQ=255) aTCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCg > 2:337180/1‑90 (MQ=255) aaTATGATCGTCTATCCAGTGATCCGCCCCCTGGTCGTAATAATCCCCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCa < 1:527042/90‑1 (MQ=255) cGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGaa > 1:174364/1‑90 (MQ=255) ccAGTGATTCCCCCCCTGGTCGTGATAACCCCCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGttt < 2:143069/90‑1 (MQ=255) gTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGt > 1:167657/1‑66 (MQ=255) gTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGt < 2:167657/66‑1 (MQ=255) gTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAAt > 1:143069/1‑90 (MQ=255) ccccTGGTCGTAATAACCCCCGCCGCCAGCGCTTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAg < 2:143053/90‑1 (MQ=255) gTCGTGATAACCCCCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCat < 1:71168/90‑1 (MQ=255) gTCGTAATACCCCCCGCCGCCAGCGCTTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCat < 2:553980/90‑1 (MQ=255) cGTAATAACCCCCGCCGCCAGCGCTTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCatga < 2:174364/90‑1 (MQ=255) tGATAACCCCCGCCGCCAGCCCTTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCatgatg < 2:141173/90‑1 (MQ=255) | CGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATG > NZ_CP009273/2354740‑2354897 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |