| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,225,956 | 0 | T | G | 52.6% | 5.4 / 13.1 | 19 | S41R (AGT→AGG) ‡ | yjbE | exopolysaccharide production protein YjbE |
| Reads supporting (aligned to +/- strand): ref base T (4/5); new base G (10/0); total (14/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.48e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCG‑CTTGCGGCAACCGGTGGC‑GGTGATGGTTCTAATAC > NZ_CP009273/4225874‑4226039 | ttGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGAGGGGAGTTCCTCcg > 1:457665/1‑90 (MQ=255) tGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGttctcccc > 1:1010175/1‑88 (MQ=255) gcggcgACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAATCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTTCTCcgcg > 1:118708/1‑90 (MQ=255) cGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTCCCCCGCGGCCAGCGgcagca > 1:994374/1‑90 (MQ=255) ggCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTCCCCCGCGACCCGCGGCAGCACCgc > 1:412101/1‑89 (MQ=255) cACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTTCTCCCCGGCCAGCGTCAGCACCGTAAACt > 1:328009/1‑90 (MQ=255) gTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTCCCCCCCGACCAGGGTCACCACCGTAAACTccgcg > 1:200153/1‑90 (MQ=255) ggTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTCCCCCGCGGCCCGCGTCCGCACCCGAAGCTCGGCcggggg > 2:588040/1‑90 (MQ=255) tGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTCCTCCCCGACCAGCGGCAGCACCGTAAGCTCGgggggggggg > 1:902422/1‑88 (MQ=255) aaGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGTTCCTCCGCGACCCGCGTCAGCACCCTAAGCTCGGCGGGGGGGGGcgcgg > 1:1532437/1‑89 (MQ=255) aaGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTCCTCCGCGACCAGCGGCAGCACCGTAAGCTCGGCGGGGGGGGgcgcg‑c > 1:1077903/1‑90 (MQ=255) cAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGGGGTTCCCCCCGACCCGCGGCCGCCCCCCAAACTCGGCGGGGGGGGGCGCGCCTTGCg > 2:1286752/1‑90 (MQ=255) gTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCG‑CTTGCGGCAACCGGTGGC‑GGTGa < 1:1013126/90‑1 (MQ=255) cGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCTCG‑CTTGCGGCAACCGGTGGC‑GGTGATg < 2:672396/90‑1 (MQ=255) cGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCG‑CTTGCGGCAACCGGTGGC‑GGTGATg < 2:994374/90‑1 (MQ=255) cGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCG‑CTTGCGGCAACCGGTGGC‑GGTGATg < 1:736345/90‑1 (MQ=255) tttCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGGGGGGGTCGCG‑CTTGCGGCAACCCGGGGC‑GGGGGTgggt > 1:856913/1‑88 (MQ=255) ggcggGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCG‑CTTGCGGCAACCGGTGGC‑GGTGATGGTTCTaa < 2:942043/90‑1 (MQ=255) ggggGTTCCTCCCCGACCAGCGTCAGCACCGTAAGCTCGGCGGGGGGGGGGCCG‑CCTGCGGCGACCCGGGGCGGGGGATGGTTTTataac > 2:305750/1‑86 (MQ=255) | TTGCGGCGACTTCTGCGTGGGCTGCACCTGTACAGGTGGGCGAAGCGGCAGGGTCGGCAGCAACGTCGGTTTCGGCGGGGAGTTCCTCCGCGACCAGCGTCAGCACCGTAAGCTCGGCGGTGGGTGTCGCG‑CTTGCGGCAACCGGTGGC‑GGTGATGGTTCTAATAC > NZ_CP009273/4225874‑4226039 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |