| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 890,386 | 0 | A | C | 52.4% | 14.6 / 17.4 | 22 | intergenic (+34/‑61) | potF/potG | spermidine/putrescine ABC transporter substrate‑binding protein PotF/putrescine ABC transporter ATP‑binding subunit PotG |
| Reads supporting (aligned to +/- strand): ref base A (9/1); new base C (0/11); total (9/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.40e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.06e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCG > NZ_CP009273/890302‑890466 | aTCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACccgc > 1:196128/1‑90 (MQ=255) gACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCgg > 2:473989/1‑90 (MQ=255) gtgCGCCCCCGCGGGGGGACCAAAGTGAAGAGGGGAAAATAATCCGCAGTCGTAGATGCCGGGGGGCCGCACCACCCCCGCCGGCAATTc < 1:449747/90‑1 (MQ=255) gcccccGCGCGTGGACCAAAGGAAAGAGCGGAAAATAACCCGCAGTCGTAGATGCCGGAGGGGCGCCCCACACCCGCCGGCAATTCGCAc < 1:1640369/87‑1 (MQ=255) gcccccGCGCGGGGCCCAAAGTGAAAGGCGGAAAAAAACCCGCGGTGGTAGACGCGGGAGGGGCCCACCCCTCCCGCCGGCAATTCGCAc < 2:937770/87‑1 (MQ=255) ccccccGCGCGTGGACCAAAGTAAAGAGCGAAAAATAACCCGCAGTCGTAGATGCCGGGGGGGCGCACCACCCCCGCCGGCAATTCGCAc < 2:1302102/87‑1 (MQ=255) cccGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCAt > 1:1431826/1‑90 (MQ=255) gcgcgTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTGGGAGATGCGGGGGGAGCGCACCACACCCACCGGCAAGGCGCACCATTAt > 2:952835/1‑90 (MQ=255) gACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTgcgc > 1:1368094/1‑90 (MQ=255) aCCAAAGTGAAGAGCGGAAAAAAACCCGCAGTCGTAGATGCGGGGGGGGCGCCCCACCCCCGCCGGCAATTCGCACCATTATGGTGCGCt < 1:216124/90‑1 (MQ=255) caaaaGTAAAGAGCGAAAAAAAACCCGCAGTCGTAGATGCCGGGGGGGCCCCCCCCCCCCGCCGGCAATTCGCACCATTATGGTGCGCtt < 2:1431826/88‑1 (MQ=255) aggaaaGAGCGAAAAATACTCCGCAGTCGAAGAGGCCGGGGGGGCGCCCCACCCCCGCCGGCAATTCGCACCATTATGGTGCGCTTGcac < 2:1450152/86‑1 (MQ=255) aaGGGCGAAAAATAACCCGCGGTGGAAGGCGCGGGGGGGGCCCCCCCCCCCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACAt < 2:957592/90‑1 (MQ=255) gagCGGAAAATACCCCGCAGTCGTAGATGCGGGAGGGGCGCCCCACCCCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTc < 1:1637593/90‑1 (MQ=255) gagCGGAAAATAACCCGCAGTCGTAGGTGCGGGGGGGGCCCCCCCCCCCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTc < 2:177536/90‑1 (MQ=255) aaaTAATCCGCAGTCGTATATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCg > 1:453117/1‑90 (MQ=255) aataacCCGCAGTCGTAGATGCCGGGGGGGCCCCCCCCCCCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCgg < 2:770299/90‑1 (MQ=255) cAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGcc > 1:1370374/1‑90 (MQ=255) tAGGTGCGGGGGGGGCCCACCCCCCCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAAt < 2:1118437/90‑1 (MQ=255) tAGAGGCCGGGGGGGCGCCCCACCCCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAAt < 2:978574/90‑1 (MQ=255) aTGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGAc > 2:1617073/1‑90 (MQ=255) gcgcACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCg > 1:1355463/1‑90 (MQ=255) | ATCGACCGTGTGCGCACCCGCGCGTGGACCAAAGTGAAGAGCGGAAAATAATCCGCAGTCGTAGATGCCGGAGGGGCGCACCACACCCGCCGGCAATTCGCACCATTATGGTGCGCTTGCACACATTCAATGCCGGAGAGCAGCCGTGAATGACGCTATCCCTCG > NZ_CP009273/890302‑890466 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |