| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,819,318 | 0 | G | A | 62.1% | ‑4.1 / 27.2 | 29 | intergenic (‑124/+79) | ves/spy | environmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone Spy |
| Reads supporting (aligned to +/- strand): ref base G (11/0); new base A (0/18); total (11/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.89e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.26e-05 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCA > NZ_CP009273/1819230‑1819406 | tCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGagaaaaga > 2:409861/1‑90 (MQ=255)tCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGagaaaaga > 2:1031174/1‑90 (MQ=255) aGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGagaaaagaaa > 2:720850/1‑90 (MQ=255) aGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGagaaaagaaa > 2:1427599/1‑90 (MQ=255) aGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGagaaaagaaa > 1:371888/1‑90 (MQ=255) gTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTc > 2:1689374/1‑90 (MQ=255) ttATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTcc > 1:325078/1‑90 (MQ=255) ttATCGCCAACTGGGGTGATTTACGTTGGGGGTGATCTGGTTTTTTTTAAAACTTTTT‑GAGAAAAAAAAAAAAAACCGCCGATCCTGTcc < 2:463383/90‑1 (MQ=255) aTCGGCAACTGGGGTGATTTACGTTGGTGGTGATCAGGTTTTTTTTACACCTGTTT‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCAc < 1:560356/90‑1 (MQ=255) tCGGCAGCGGGGGTATTTTGCCTTAGTGGTGAGCAGTTTTTTTTTACAACTGTTT‑GAAAAGAAAAAAAAAACCCGCCGATCCTGTCCAcc < 1:1354308/90‑1 (MQ=255) ccGGCACCGCGGGTTTTTTACGTTAGTGGTAATCAGTTTTTTTTTAAAATTTTTT‑GAAAAAAAAAAAAAAAACCGCCGATCCTGTCCAcc < 2:1146184/89‑1 (MQ=255) cAACTGGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCAt < 1:543050/90‑1 (MQ=255) aaCTGGGGTGTTTTACGTTAGTGGTGATCAGGTTTTTTTTAAAATTTTTT‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCAtt < 1:1194774/90‑1 (MQ=255) aaCTGGGGTGATTTACGTTAGGGGTGATCAGGTCTTTTTTACAACTGTTT‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCAtt < 1:1031174/90‑1 (MQ=255) aaCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCAtt > 1:854916/1‑90 (MQ=255) aCTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTa > 2:980033/1‑90 (MQ=255) gtgtTTTACTTTAGTGGTGATCAGGTTTTTTTTACAACTTTTT‑GAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 1:1689374/90‑1 (MQ=255) gtaaTTTACTTTAGTGGTGATCAGGTTTTTTTTACAACTTTTT‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCaa < 1:151286/87‑1 (MQ=255) ttaTTTACTTTAGTGGTGATCAGGTTTTTTTTCCAATTGTTT‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCCCCGCATTACTGCAAg < 1:105560/88‑1 (MQ=255) ttccGTTAGTGGTGATCAGTTTTTTTTAACAGTTTTTG‑GAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAg < 2:371888/87‑1 (MQ=255) ttACTTTAGTGGTGCTCTGCTTTTTTTTAAAATTTTTT‑GGGAAGAGAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAg < 2:600307/90‑1 (MQ=255) ttACGTTGGTGGTGATC‑GGTTTTTTTTAAAAATTTTTGGAAAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAg < 2:1261758/90‑1 (MQ=255) tACGTTAGTGGTGATCGGGTTTTTTTTACATTTTTTT‑GAAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGt < 2:1014265/90‑1 (MQ=255) cGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGAAgctg > 1:1361914/1‑87 (MQ=255) gTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGa > 2:151286/1‑90 (MQ=255) gATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg > 2:946116/1‑90 (MQ=255) gATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg > 2:794468/1‑90 (MQ=255) aTCAGGTTTTTTTTACAACTGTTA‑GAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc < 2:854916/90‑1 (MQ=255) aTCAGGTTTTTTTAACACCTTTTA‑AAAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc < 2:1480757/90‑1 (MQ=255) aaattGTTT‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttttt < 1:1067112/86‑1 (MQ=255) gTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCTCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTgggtt > 1:839594/1‑87 (MQ=255) gTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTg > 2:1123585/1‑90 (MQ=255) gaaaagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAg < 2:325078/87‑1 (MQ=255) agagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGCTTAAGTTTTTTGGCGGAAAGatta > 2:514755/1‑90 (MQ=255) agaaaagaaaACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGattatt > 2:1734824/1‑90 (MQ=255) agaaaagaaaACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGattatt > 2:1473280/1‑90 (MQ=255) aagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagc < 1:1549449/90‑1 (MQ=255) agaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagca < 1:1123585/90‑1 (MQ=255) | TCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCA > NZ_CP009273/1819230‑1819406 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |