Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,912	G→T	W30C (TGG→TGT)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,912	0	G	T	100.0%	179.4 / NA	67	W30C (TGG→TGT)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (28/39);  total (28/39)

GATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAA  >  NZ_CP009273/49825‑49996                                                                                        |                                                                                     gATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGttt                                                                                    <  1:69162/90‑1 (MQ=255) gATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGttt                                                                                    <  1:445533/90‑1 (MQ=255) gATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGttt                                                                                    <  1:151496/90‑1 (MQ=255)   tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTa                                                                                  <  1:1393449/90‑1 (MQ=255)   tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTa                                                                                  >  1:152154/1‑90 (MQ=255)    cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACTCCATGCCGTGGAAGCCGCCTGCCGATCTCGCCTGTTTTaa                                                                                 >  2:281205/1‑90 (MQ=255)     aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTaaa                                                                                >  1:1485324/1‑90 (MQ=255)       tCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACg                                                                              >  1:747115/1‑90 (MQ=255)          gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGTATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                           <  1:1032550/90‑1 (MQ=255)          gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                           <  2:1452575/90‑1 (MQ=255)          gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                           <  1:879573/90‑1 (MQ=255)          gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                           <  1:905364/90‑1 (MQ=255)          gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                           <  1:500745/90‑1 (MQ=255)          gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                           <  1:195943/90‑1 (MQ=255)          gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                           <  1:1782905/90‑1 (MQ=255)          gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                           <  2:1289808/90‑1 (MQ=255)          gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                           <  2:937433/90‑1 (MQ=255)          gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                           <  2:1485324/90‑1 (MQ=255)          gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                           <  2:49517/90‑1 (MQ=255)          gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa                                                                           <  1:1192522/90‑1 (MQ=255)          gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACCCCATGCCGGGGAACCTCCCTGCCGATCCCGCCTGTTTTAAACGCaa                                                                           <  1:544345/90‑1 (MQ=255)           aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAac                                                                          <  1:833589/90‑1 (MQ=255)           aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAac                                                                          >  1:700844/1‑90 (MQ=255)           aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAac                                                                          >  2:1707542/1‑90 (MQ=255)             tGCGGCGTTAGCGGTAGATCGCTTTACCGGCATGGAAACCCCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAacac                                                                        <  2:1074383/90‑1 (MQ=255)             tGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAacac                                                                        <  2:1679525/90‑1 (MQ=255)                ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCtt                                                                     >  1:910625/1‑90 (MQ=255)                ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCtt                                                                     >  1:1548258/1‑90 (MQ=255)                  cgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAAc                                                                               >  2:1043071/1‑78 (MQ=255)                  cgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAAc                                                                               <  1:1043071/78‑1 (MQ=255)                     tAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTtaaata                                                                <  1:1707542/90‑1 (MQ=255)                        cGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAAc                                                             >  2:1248620/1‑90 (MQ=255)                          gTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAAccc                                                           <  1:1505679/90‑1 (MQ=255)                           tAGATCGCGTTATCGGCATGGAAAACGCCATGCCGGGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCg                                                          >  2:510463/1‑90 (MQ=255)                            aGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGt                                                         >  1:836859/1‑90 (MQ=255)                            aGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGt                                                         <  1:1334382/90‑1 (MQ=255)                             gATCGCGTTATCGGCATGGAAAACGCCATGTCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTg                                                        >  2:896221/1‑90 (MQ=255)                             gATCGCGTTATCGGCATGGAAAACGCCATGCCGGGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTg                                                        >  2:968818/1‑90 (MQ=255)                             gATAGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGACGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTg                                                        <  1:1189379/90‑1 (MQ=255)                                 gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                                    >  1:534955/1‑90 (MQ=255)                                 gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                                    >  1:1472862/1‑90 (MQ=255)                                 gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAAAGCAACACCTTAAATAAACCCGTGATTa                                                    >  2:304230/1‑90 (MQ=255)                                      aTCGGCATGGAAACCGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgc                                               <  1:106197/90‑1 (MQ=255)                                      aTCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgc                                               >  2:75228/1‑90 (MQ=255)                                      aTCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgc                                               <  1:1012419/90‑1 (MQ=255)                                       tCGCCAGGGAAACCCCCATGCCGGGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgcc                                              <  2:836859/90‑1 (MQ=255)                                            aTGAAAACCCCCATGCCGTGAACCCTCCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                                         <  2:1472862/90‑1 (MQ=255)                                              ggAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTaa                                                                                 >  1:1046143/1‑48 (MQ=255)                                              ggAAAACCCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTaa                                                                                 <  2:1046143/48‑1 (MQ=255)                                                    cGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAac                                                                          >  2:516521/1‑49 (MQ=255)                                                    cGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAac                                                                          <  1:516521/49‑1 (MQ=255)                                                    cGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCt                                                                      <  1:15131/53‑1 (MQ=255)                                                    cGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCt                                                                      >  2:15131/1‑53 (MQ=255)                                                        aTGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca                             >  2:960345/1‑90 (MQ=255)                                                         tGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCa                                                                            >  1:693058/1‑42 (MQ=255)                                                         tGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCa                                                                            <  2:693058/42‑1 (MQ=255)                                                         tGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGt                                                         <  2:217376/61‑1 (MQ=255)                                                         tGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGt                                                         >  1:217376/1‑61 (MQ=255)                                                         tGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaa                            >  2:1559863/1‑90 (MQ=255)                                                             gTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCgg                        >  1:1755204/1‑90 (MQ=255)                                                               ggACCCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc                      <  2:463936/90‑1 (MQ=255)                                                               ggACCCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc                      <  1:529380/90‑1 (MQ=255)                                                                   cttccctgccGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTcc                  <  1:93966/86‑1 (MQ=255)                                                                     tgcctgccGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt                <  1:1704834/90‑1 (MQ=255)                                                                          gccGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc                                            <  2:451597/57‑1 (MQ=255)                                                                          gccGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc                                            >  1:451597/1‑57 (MQ=255)                                                                              aTCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc       <  2:304167/90‑1 (MQ=255)                                                                                ctcGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGc     >  2:1617927/1‑90 (MQ=255)                                                                                ctcCCCTGTTTTAAACGCAACCCCTTAAATAAACCCGGGCTTATGGGCCGCCATAACTGGGAATCAATCGGTCGTCCGTTGCCAGGACGc     <  2:771085/90‑1 (MQ=255)                                                                                   gCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaaa  <  1:1406283/90‑1 (MQ=255)                                                                                        |                                                                                     GATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAA  >  NZ_CP009273/49825‑49996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑