Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,613,383 Δ1 bp coding (7/435 nt) marR → multiple antibiotic resistance transcriptional regulator MarR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,613,3800A.85.7% 32.9 / 3.5 14coding (4/435 nt)marRmultiple antibiotic resistance transcriptional regulator MarR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (6/6);  minor base T (1/1);  total (7/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGC  >  NZ_CP009273/1613326‑1613461
                                                      |                                                                                 
tGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTG‑AAAGTACCAGCGATCTGTTCAATGAAATTATTCCAt                                               >  2:457189/1‑90 (MQ=255)
 gCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTG‑AAAGTACCAGCGATCTGTTCAATGAAATTATTCCAtt                                              >  2:380263/1‑90 (MQ=255)
     gggCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTG‑AAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGt                                          <  2:146027/90‑1 (MQ=255)
                 ccccTGCAACTAATTACTTGCCAGGGCAACTAATGTG‑AAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCat                              <  1:360928/90‑1 (MQ=255)
                    cTGCAACTAATTACTTGCCAGGGCAACTAATGTG‑AAAGTACCAGCg                                                                       <  1:157725/46‑1 (MQ=39)
                    cTGCAACTAATTACTTGCCAGGGCAACTAATGTG‑AAAGTACCAGCg                                                                       >  2:157725/1‑46 (MQ=39)
                    cTGCAACTAATTACTTGCCAGGGCAACTAATGTG‑AAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTa                                    >  1:337298/1‑81 (MQ=255)
                    cTGCAACTAATTACTTGCCAGGGCAACTAATGTG‑AAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTa                                    <  2:337298/81‑1 (MQ=255)
                    cTGCAACTAATTACTTGCCAGGGCAACTAATGT‑TAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCat                              >  1:26277/1‑87 (MQ=255)
                    cTGCAACTAATTACTTGCCAGGGCAACTAATGT‑TAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCat                              <  2:26277/87‑1 (MQ=255)
                                aCTTGCCAGGGCAACTAATGTG‑AAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCagaag               <  1:457189/90‑1 (MQ=255)
                                 cTTGCCAGGGCAACTAATGTG‑AAAGTACCAGCGATCTGTTCAATGAAattat                                                    <  2:199269/52‑1 (MQ=255)
                                 cTTGCCAGGGCAACTAATGCG‑AAAGTACCAGCGATCTGTTCAATGAAattat                                                    >  1:199269/1‑52 (MQ=255)
                                             aCTAATGTG‑AAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGc  >  1:261682/1‑90 (MQ=255)
                                                      |                                                                                 
TGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGC  >  NZ_CP009273/1613326‑1613461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑