| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 333,780 | 0 | A | G | 56.2% | 6.8 / 12.3 | 16 | D431G (GAT→GGT) | fdrA | acyl‑CoA synthetase FdrA |
| Reads supporting (aligned to +/- strand): ref base A (3/4); new base G (9/0); total (12/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.92e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCC > NZ_CP009273/333716‑333859 | accaACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGGGATCGTGATGGGTTTTTTGGTCGGGTTTGTATCGCAt > 1:384179/1‑90 (MQ=255) aCCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGGTGGGTTTTTTGCTCGGGTTTTGATCGCATGAAg > 1:400679/1‑90 (MQ=255) aCCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGGGGTCGTGGTGGGGTTTTTGTTCGGGGTTTGATCGCATGAAg > 1:220340/1‑90 (MQ=255) tCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGGGGTAGTGGTGGGTTTTGTGGTCGGGTTTTGGTCGCCTGAAGAACCGGGCgg > 1:107506/1‑90 (MQ=255) tCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGGGGTAGGGGTGGGTTTTTTGGTCGGGTTTGGGTCGCCTGAAGATgcgggcgg > 1:219034/1‑90 (MQ=255) tCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGGGATCGGGGTGGGTTTTTTGGTCGGGTTTGGGTCGCATGAAGATCCCGTCgg > 1:348041/1‑90 (MQ=255) cGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGc < 1:297170/90‑1 (MQ=255) gagGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCgag < 2:220340/90‑1 (MQ=255) gagGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCgag < 2:400679/90‑1 (MQ=255) gagGCGCGCGATCCAGAAGTGGCGGGGGTTGTGGTGGGTTTTGTGGTCGGGTTTTGGTCGCATGAAGAACCGGGCGGCGCCACCATCgag > 1:238309/1‑90 (MQ=255) gcgcgATCCAGAAGTGGCGGGGGTCGGGATGGGTTTTTGGCTCGGGGTTTGGTCGCCTTAAGAAACGGGCGGCTCCCCCAGCCAAACGAt > 2:446530/1‑90 (MQ=255) ggCGGTGGTCGTGATGGGTTTTTTGCTCGGGTTTTGATCGCATGAAGATCCGGGCGGGTCCCCCCTCGCGGCGATCAAAAaagggaaaac > 1:440004/1‑88 (MQ=255) ggCGGTGGTCGTGATGGATTTTTTGGTCGGGTTTGGGGCGGATGGAGAGCCGGTCGGCCCCCCCCTCGTGACGATCCAAAaaacggaaac > 1:195534/1‑88 (MQ=255) ggCGGTGATCGTGGTGGATTTTTTGCTCGGATTTGGGTCGCATGAAGATCCGGGCGGGTCCCCCATCGAGACGATCAAAGaagcggaagg > 1:139398/1‑89 (MQ=255) ggCGGTGATCGTGATGGATTTTTTGCTCGGATTTGGATCGCATGAAGAACCGGGCGGCTCCACCATCGAGACGATCAAAGaaaggaaaac > 2:175515/1‑88 (MQ=255) aTCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATcgcc < 1:175515/90‑1 (MQ=255) | ACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCC > NZ_CP009273/333716‑333859 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |