| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 603,218 | 0 | C | A | 63.6% | ‑3.8 / 12.1 | 11 | intergenic (‑379/‑74) | ybdK/hokE | YbdK family carboxylate‑amine ligase/type I toxin‑antitoxin system toxin HokE |
| Reads supporting (aligned to +/- strand): ref base C (2/2); new base A (3/4); total (5/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.60e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGGGTAAGATAACGGCGGGTGCCTGAAGCTTTCCGGTTTCAGGTTTACTCTGAGGTCTGGAAAGATGAAGCCCCAGGAGATATTTCTATCAACCCTGGGGCTGCCACTCCAAACCCGAACAATTTGGATGGTAGTCCCTTCTTCGCATGGAGGCAATATAAACATGCTGAC > NZ_CP009273/603129‑603299 | aGGGTAAGATAACGGCGGGTGCCTGAAGCTTTCCGGTTTCAGGTTTACTCTGAGGTCTGGAAAGATGAAGCCCCAGGAGATATTTCTATc < 1:204858/90‑1 (MQ=255) cggGTGCCTGAAGCTTTCCGGTTTCAGGTTTACTCTGAGGTCTGGAAAGATGAAGCCCCAGGAGATATTTCTATAAACCCTGGGGCTGcc < 2:14200/90‑1 (MQ=255) ctGAGGTCTGGAAAGATGAAGCCCCAGGAGATATTTCTATCAACCCTGGGGCTGCCACTCCAAACCCGAACAATTTGGATGGTAGTCCct > 1:363344/1‑90 (MQ=255) cTGGAAAGATGAAGCCCCAGGAGATATTTCTATAACCCCTGGGGCTGCCACTCCAAAc < 1:264899/58‑1 (MQ=255) cTGGAAAGATGAAGCCCCAGGAGATATTTCTATAAACCCTGGGGCTGCCACTCCAAAc < 1:112877/58‑1 (MQ=255) cTGGAAAGATGAAGCCCCAGGAGATATTTCTATAAACCCTGGGGCTGCCACTCCAAAc > 2:112877/1‑58 (MQ=255) cTGGAAAGATGAAGCCCCAGGAGATATTTCTATAAACCCTGGGGCTGCCACTCCAAAc > 2:264899/1‑58 (MQ=255) aTGAAGCCCCAGGAGATATTTCTATCAACCCTGGGGCTGCCACTCCAAACCCGAACAATTTGGATGGTAGTCCCTTCTTCGCATGGAGGc < 2:158279/90‑1 (MQ=255) aGCCCCAGGAGATATTTCTATAAACCCTGGGGCTGCCACTCCAAACCCGAACAATTTGGATGGTAGTCACTTCTTCGCATGGAGGCAata > 2:306271/1‑90 (MQ=255) tatTTCTATCAACCCTGGGGCTGCCACTCCAAACCCGAACAATTTgg < 1:99469/47‑1 (MQ=255) tatTTCTATCAACCCTGGGGCTGCCACTCCAAACCCGAACAATTTgg > 2:99469/1‑47 (MQ=255) atTTCTATAAACCCTGGGGCTGCCACTCCAAACCCGAACAATTTGGATGGTAGTCACTTCTTCGCATGGAGGCAATATAAACATGCTGAc < 1:306271/90‑1 (MQ=255) | AGGGTAAGATAACGGCGGGTGCCTGAAGCTTTCCGGTTTCAGGTTTACTCTGAGGTCTGGAAAGATGAAGCCCCAGGAGATATTTCTATCAACCCTGGGGCTGCCACTCCAAACCCGAACAATTTGGATGGTAGTCCCTTCTTCGCATGGAGGCAATATAAACATGCTGAC > NZ_CP009273/603129‑603299 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |