| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,164,017 | 2 | . | C | 50.0% | 7.5 / 10.8 | 8 | coding (272/633 nt) | comR | TetR family copper‑responsive transcriptional repressor ComR |
| Reads supporting (aligned to +/- strand): ref base . (2/2); new base C (2/2); total (4/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.68e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGTAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTT‑‑TCTCTTCACAAAACAGCTGTGCTTCATGCTTAGCGGCAAAACGATCGATATAGCGGTCGAGAACGGCACGAA > NZ_CP009273/1163928‑1164089 | tgATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTTGTAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCttt > 1:168530/1‑90 (MQ=255) cATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGTAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTT‑‑tctc < 1:161773/90‑1 (MQ=255) tgtCTTTGCTGGTAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTT‑‑TCTCTTCACAAAACAGCTGTGCtt > 1:130705/1‑90 (MQ=255) gTAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTCTTCACAAAACAGCTGTGCTTCATGCTTAg > 2:304602/1‑90 (MQ=255) gTAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTTTCTCTCTTCACAAAACAGCTGTGCTTCATGCTTAg > 2:374363/1‑90 (MQ=255) cagccagcGCAGACTCCACGCTTT‑‑TCTCTTCACAAAACAGCTGTGCTTCATGCTTAGCGGCaa < 1:107945/63‑1 (MQ=255) cagccagcGCAGACTCCACGCTTT‑‑TCTCTTCACAAAACAGCTGTGCTTCATGCTTAGCGGCaa > 2:107945/1‑63 (MQ=255) ccagcGCAGACTCCACGCTTT‑‑TCTCTTCACAAAACAGCTGTGCTTCATGCTTAGCGGCAAAATGATCGATATAGCGGTCGAGAACGGCAc < 2:168530/90‑1 (MQ=255) gcAGACTCCACGCTTTTCTCTCTTCACAAAACAGCTGTGCTTCATGCTTAGCGGCAAAACGATCGATATAGCGGTCGAGAACGGCACGaa < 1:304602/90‑1 (MQ=255) gcAGACTCCACGCTTTTCTCTCTTCACAAAACAGCTGTGCTTCATGCTTAGCGGCAAAACGATCGATATAGCGGTCGAGAACGGCACGaa < 1:374363/90‑1 (MQ=255) | TGATCATGAAGCAGCCTGCCGGGGTGTCTTTGCTGGTAAAGCAGTTGGCGATGGCAGCAAAATAGTCAGCCAGCGCAGACTCCACGCTTT‑‑TCTCTTCACAAAACAGCTGTGCTTCATGCTTAGCGGCAAAACGATCGATATAGCGGTCGAGAACGGCACGAA > NZ_CP009273/1163928‑1164089 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |