| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,315,387 | 0 | G | T | 53.8% | 5.2 / 20.7 | 13 | L85L (CTC→CTA) | trpD | bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD |
| Reads supporting (aligned to +/- strand): ref base G (4/2); new base T (4/3); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCGCCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGTGAGGAGTTCCGGCATACAACCGGCTTC > NZ_CP009273/1315299‑1315455 | cATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCGCCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGa < 2:138940/90‑1 (MQ=255) aTTTCGCCCGCCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCaa < 1:411093/63‑1 (MQ=255) aTTTCGCCCGCCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCaa > 2:411093/1‑63 (MQ=255) gccTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGTg > 1:180722/1‑90 (MQ=255) cccccGTAAGCTTCGACAATCGCCTGATGACCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGTGAGGAGTTCCGGCATa > 2:38864/1‑90 (MQ=255) accccGTAAGCTTCGACAATCGCCTGATGTCCTAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGTGAGGAGTTCCGGCATa > 2:392464/2‑90 (MQ=255) ccGTAAGCTTCGACAATCGCCTGATGTCCTAGGCaa > 1:186129/1‑36 (MQ=37) ccGTAAGCTTCGACAATCGCCTGATGTCCTAGGCaa > 1:283554/1‑36 (MQ=37) ccGTAAGCTTCGACAATCGCCTGATGTCCTAGGCaa > 1:90002/1‑36 (MQ=255) ccGTAAGCTTCGACAATCGCCTGATGTCCTAGGCaa < 2:186129/36‑1 (MQ=37) ccGTAAGCTTCGACAATCGCCTGATGTCCTAGGCaa < 2:283554/36‑1 (MQ=37) ccGTAAGCTTCGACAATCGCCTGATGTCCTAGGCaa < 2:90002/36‑1 (MQ=37) ttCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGTGAGGAGTTCCGGCATACAACCGGCTTc > 2:392600/1‑90 (MQ=255) | CATGTTCAATGCTGGAGGCTTTACCGTGGAGAATTTCGCCCGCCTGACCGACATAGCCCCCGTAAGCTTCGACAATCGCCTGATGTCCGAGGCAAATGCCAATAATGGGCAGCTTGCCACGCAAGCGGGTGAGGAGTTCCGGCATACAACCGGCTTC > NZ_CP009273/1315299‑1315455 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |