| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,102,255 | 0 | T | C | 53.8% | 1.5 / 13.6 | 13 | E87G (GAG→GGG) | speC | ornithine decarboxylase |
| Reads supporting (aligned to +/- strand): ref base T (6/0); new base C (0/7); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTGGCAGCAAATTCTCTTCATACTGACAGGCTGCGGATTCCAGCTCCAGCCACTGCTGCTCGTTGCCGTTGATTACCGCCGTAACGCCCGCAGGTAATTCAACAGCATGTTCGGAATACAAAAACACCGGTA > NZ_CP009273/3102167‑3102342 | gCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTGGCAGCAAATTCTCTTCATACTGACAGGCTGCGGATtccagctc < 1:7778/90‑1 (MQ=255) cTGCGTCAGCGTGTCATAAAACGGTGGCAGCAAATTCTCTTCATACTGACAGGCTGCGGATTCCAGCTCCAGCCACTGCTGCTCGTTGcc > 1:286171/1‑90 (MQ=255) tGCGTCAGCGTGTCATAAAACGGTGGCAGCAAATTCTCTTCATACTGACAGGCTGCGGATTCCAGCTCCAGCCACTGCTGCTCGTTGCCg > 1:101893/1‑90 (MQ=255) tGCGTCAGCGTGTCATAAAACGGTGGCAGCAAATTCTCTTCATACTGACAGGCTGCGGATTCCAGCTCCAGCCACTGCTGCTCGTTGCCg > 1:66109/1‑90 (MQ=255) aGCGTGTCATAAAACGGTGGCAGCAAATTCTCTTCATACTGACAGGCTGCGGATTCCAGCTCCAGCCACTGCTGCTCGTTGCCGTTGAtt > 1:69181/1‑90 (MQ=255) aaCGGTGGCAGCAATTTCTCTTCATCCTGACAGGCTGCGGATTCCAGCCCCAGCCACTGCTGCTCGTTGCCGTTGATTACCGCCGTAACg < 2:202051/90‑1 (MQ=255) gTGGCAGCAAATTCTCTTCATACTGACAGGCTGCGGATTCCAGCTCCAGCCACTGCTGCTCGTTGCCGTTGATTACCGCCGTAACGCCCg > 1:384029/1‑90 (MQ=255) gcagaaATTTCTTTTCATACTGACAGGCGGGGGATCCCAGCCCCAGCCACTGCTGCTCGTTGCCGTTGATTACCGCCGTAACGCCCGCAg < 2:69181/90‑1 (MQ=255) agaaaaTTCTTTTCATACTGACAGGCTGGGGATTCCAGCCCCAGCCACTGCTGCTCGTTGCCGTTGATTACCGCCGTAACGCCCGCAGGt < 2:384029/87‑1 (MQ=255) tctcTTCATACTGACGGGCGGCGGATTCCAGCCCCAGCCACTGCTGCTCGTTGCCGTTGATTACCGCCGTAACGCCCGCAGGTAATTCaa < 2:466103/90‑1 (MQ=255) ttCATACTGACAGGCTGCGGATTCCAGCTCCAGCCACTGCTGCTCGTTGCCGTTGATTACCGCCGTAACGCCCGCAGGTAATTCAACAGc > 2:333540/1‑90 (MQ=255) cTGACGGGCTGCGGATCCCAGCCCCAGCCCCTGCTGCTCGTTGCCGTTGATTACCGCCGTAACGCCCGCAGGTAATTCAACAGCATGTTc < 2:66109/90‑1 (MQ=255) cTGACGGGCGGGGGTTTCCAGCCCCAGCCACTGCTGCTCGTTGCCGTTGTTTACCGCCGTAACGCCCGCAGGTAATTCAACAGCATGTTc < 2:101893/90‑1 (MQ=255) cAGGCGGGGGTTCCCAGCCCCAGCCACTGCTGCTCGTTGCCGTTGATTACCGCCGTAACG‑CCGCAGGTAATTCAACAGCATGTTCGGAAt < 2:286171/90‑1 (MQ=255) gctccagcCACTGCTGCTCGTTGCCGTTGATTACCGCCGTAACGCCCGCAGGTAATTCAACAGCATGTTCGGAATACAAAAACACCGGTa < 1:333540/90‑1 (MQ=255) | GCTGTTGCCCATCTCAACGTACTGCGTCAGCGTGTCATAAAACGGTGGCAGCAAATTCTCTTCATACTGACAGGCTGCGGATTCCAGCTCCAGCCACTGCTGCTCGTTGCCGTTGATTACCGCCGTAACGCCCGCAGGTAATTCAACAGCATGTTCGGAATACAAAAACACCGGTA > NZ_CP009273/3102167‑3102342 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |