| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,819,986 | 0 | G | T | 63.6% | ‑4.7 / 11.2 | 11 | D473Y (GAC→TAC) | recG | ATP‑dependent DNA helicase RecG |
| Reads supporting (aligned to +/- strand): ref base G (3/1); new base T (4/3); total (7/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAG > NZ_CP009273/3819906‑3820075 | cGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGc > 1:1671/1‑90 (MQ=255) cgcccgccGGTCCCTCCGGTCGTTATTCCTAATCCCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGgtcgt < 2:243756/90‑1 (MQ=255) gTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATAc > 2:298174/1‑90 (MQ=255) tACCCGCCGTACCGACATCATTGACCGCGTGcacc > 2:127406/1‑35 (MQ=255) cgccgTACCGACATCATTTACCGCGTGCACCACGcc > 1:200237/1‑36 (MQ=38) cgccgTACCGACATCATTTACCGCGTGCACCACGcc > 1:206650/1‑36 (MQ=38) cgccgTACCGACATCATTTACCGCGTGCACCACGcc > 1:255809/1‑36 (MQ=38) cgccgTACCGACATCATTTACCGCGTGCACCACGcc < 2:200237/36‑1 (MQ=255) cgccgTACCGACATCATTTACCGCGTGCACCACGcc < 2:206650/36‑1 (MQ=255) cgccgTACCGACATCATTTACCGCGTGCACCACGcc < 2:255809/36‑1 (MQ=255) cgccgTACCGACATCATTTACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCg > 1:394596/1‑90 (MQ=255) gACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAg > 1:167213/1‑90 (MQ=255) gACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAg > 1:238854/1‑90 (MQ=255) | CGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAG > NZ_CP009273/3819906‑3820075 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |