| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,594,167 | 0 | C | T | 57.1% | ‑1.8 / 22.4 | 21 | S64F (TCT→TTT) | bglJ | DNA‑binding transcriptional activator BglJ |
| Reads supporting (aligned to +/- strand): ref base C (0/9); new base T (12/0); total (12/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.40e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.26e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATT > NZ_CP009273/4594088‑4594252 | cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATgaga > 2:155078/1‑90 (MQ=255) ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCATTAGAAGTGAGCGCa > 1:27089/1‑90 (MQ=255) aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCcgaggg > 2:137061/1‑88 (MQ=255) tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCAGAGAggg > 1:106894/1‑90 (MQ=255) tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCAGAGAggg > 1:432233/1‑90 (MQ=255) tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCAGAGAggg > 2:468936/1‑90 (MQ=255) tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTGCCCTGAGAAGATAGGGCagggaggg > 2:6298/1‑90 (MQ=255) cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGa < 1:155078/90‑1 (MQ=255) gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCCTGGGGAGGGAGGGCAGAGggggggtt > 1:388949/1‑86 (MQ=255) gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGGTTa > 2:38326/1‑90 (MQ=255) tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGc < 1:137061/90‑1 (MQ=255) tGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGGGGGGTTATCTTGCctgactga > 2:464008/1‑90 (MQ=255) ccATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGGGGCGGGGGGGGTTTTCTTTCCTGATTGaaa > 1:82417/1‑89 (MQ=255) cATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACt < 1:245402/90‑1 (MQ=255) cATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTCCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACt < 1:353600/90‑1 (MQ=255) tcGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGGGAAGGGGGCGCAGGGGGGGGGTTTTTTTCCTGACTGAACTGGGGGTTTAGttt > 1:236908/1‑90 (MQ=255) cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCTATTAAGTTTCCGCGTAccc < 1:407492/90‑1 (MQ=255) cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCTATTAAGTTTCCGCGTAccc < 1:386581/90‑1 (MQ=255) cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAcac < 2:388949/90‑3 (MQ=255) tCATTTTTTCTTTTTCTGCCATGAGAAGTTAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCCCGTACCCGGc < 1:374549/90‑1 (MQ=255) tCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGc < 2:82417/90‑1 (MQ=255) ttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTAtt < 1:163697/90‑1 (MQ=255) | CTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATT > NZ_CP009273/4594088‑4594252 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |