Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,184,613	A→T	I207N (ATC→AAC)	phoQ ←	two‑component system sensor histidine kinase PhoQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,184,613	0	A	T	100.0%	14.0 / NA	8	I207N (ATC→AAC)	phoQ	two‑component system sensor histidine kinase PhoQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (3/5);  total (3/5)

GGTTATGTTCTTCCAGTTCGCGGACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGATCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGAACTTTTTAGCTCCACCGGAA  >  NZ_CP009273/1184527‑1184691                                                                                       |                                                                               gtttATGTTCTTCCAGTTCGCGGACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCa                                                                             <  2:284943/88‑1 (MQ=255)               aGTTCGCGGACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTg                                                               >  2:200634/1‑90 (MQ=255)                  tCGCGGACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCt                                                            >  2:56388/1‑90 (MQ=255)                                   cAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATaaa                                           >  1:11353/1‑90 (MQ=255)                                     gggCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAAcc                                         <  1:200634/90‑1 (MQ=255)                                                gggCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGAcc                              <  1:284186/90‑1 (MQ=255)                                                gggCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGAca                              <  2:11353/90‑2 (MQ=255)                                                                            acaGCAGCGGGTTCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGAACTTTTTAGCTCCACCGGaa  <  1:86175/90‑1 (MQ=255)                                                                                       |                                                                               GGTTATGTTCTTCCAGTTCGCGGACTTCTTTTGCCAGGGCTTCGATGGGGCGTAAACTCCACCAGGCGGCGACCCACAGCAGCGGGATCACTAACAGCAGATTGGCTGAGAGCACATAGATAAACCAGCTCCAGACCATATAGGAACTTTTTAGCTCCACCGGAA  >  NZ_CP009273/1184527‑1184691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑