| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,860,528 | A→T | I128N (ATC→AAC) | rpoS ← | RNA polymerase sigma factor RpoS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,860,528 | 0 | A | T | 100.0% | 34.3 / NA | 16 | I128N (ATC→AAC) | rpoS | RNA polymerase sigma factor RpoS |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (8/8); total (8/8) | |||||||||||
ATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGC > NZ_CP009273/2860462‑2860614 | aTCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGTTCAGCCCCAGGTTGCCCTCTTCg > 1:93843/1‑90 (MQ=255) cGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGTTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAgaa < 2:93843/90‑3 (MQ=255) aTGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGTTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACg < 1:442880/90‑1 (MQ=255) gTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGTTCAGCCACAGGTTGCCCTCTTCGATAAGGTCAAGCAACGcc > 1:297940/1‑90 (MQ=255) ggAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGTTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGAt > 1:117107/1‑90 (MQ=255) cGTTCCGGGTCAAACTTCTCTACCGCGCGGTTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGAtt < 1:177533/83‑1 (MQ=255) cGTTCCGGGTCAAACTTCTCTACCGCGCGGTTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGAtt > 2:177533/1‑83 (MQ=255) gggTCAAACTTCTCTACCGCGCGGTTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAAcgg > 1:168593/1‑87 (MQ=255) gggTCAAACTTCTCTACCGCGCGGTTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAAcgg < 2:168593/87‑1 (MQ=255) ccGCGCGGTTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTAccacca < 1:65835/90‑1 (MQ=255) gcgcgGTTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGAc < 1:337762/52‑1 (MQ=255) gcgcgGTTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGAc > 2:337762/1‑52 (MQ=255) gcgcgGTTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGGAATTTTTTTCACCCGa > 2:350350/1‑90 (MQ=255) gcgGTTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTTCCACCAGACg > 1:361045/1‑90 (MQ=255) gcgGTTCAGCCACAGGTTGCCCTCTTCGATAAGGTCAAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACg < 2:297940/90‑1 (MQ=255) cgGTTCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGc < 2:361045/90‑1 (MQ=255) | ATCCACCAGGTTGCGTATGTTGAGAAGCGGAAACCACGTTCCGGGTCAAACTTCTCTACCGCGCGGATCAGCCCCAGGTTGCCCTCTTCGATAAGGTCCAGCAACGCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGC > NZ_CP009273/2860462‑2860614 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |