| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 510,080 | 0 | A | C | 57.1% | 7.4 / 12.8 | 14 | V79G (GTG→GGG) | ybbJ | NfeD family protein |
| Reads supporting (aligned to +/- strand): ref base A (6/0); new base C (0/8); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCC > NZ_CP009273/510002‑510158 | gaTTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGAc > 1:154291/1‑90 (MQ=255) tcccaccaAAAACCGCCGGCCAATCAGCTGCTCCCCGCGCTGTTTAAAATGACTATCGCTGTGTTTTTGTTCGCGCCCCCGCCGCGACaa < 2:376285/88‑1 (MQ=255) tcccAGCAAAAAACGGCGGCAAATCAGCTGCCCCCCGCGCTGGTTAAAATGCCTACCGCTGTGTTTTTGTCCGCGCCCCCGCCGCGACaa < 2:318622/88‑1 (MQ=255) tcccAACACAAACCGCCGCCAAACCAGCTCCCCCCCGCGCTGTTTAAAATGCCTACCGCTGTGTTTTTGTTCGCCCCCCCGCCGCGACaa < 1:607540/88‑1 (MQ=255) cAGCAAAAACCGTCGGCAAATCAGCTGCTCCCCGCGCTGTTTAAAATGACTATCGCTGTGTTTTTTTTCGCGCCCCCGCCGCGACAACCa < 2:154291/90‑1 (MQ=255) caAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTcc > 1:66275/1‑90 (MQ=255) cGTCGCCCAACCAGCTCCCCCCCGCGTTGTTTAAAATGACTCCCGTTGTGTTTTTGTCCCCGCCCCCGCCGCGACAACCATTTCcaccac < 1:547196/90‑1 (MQ=255) gCCAACCAGCTGCCCCCCGCGCTGTTTAAAATGACTACCGCTGTGTTTTTGTTCGCCCCCCCGCCGCGACAACCATTTCCACCACAGCCa < 2:57590/90‑1 (MQ=255) taaaaTGCCTCCCGCTGTTTTTTTGTCCGCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCaa < 1:223854/88‑1 (MQ=255) ccTATCGCTGTTTTTTTTTCCCCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTAc < 1:51990/89‑1 (MQ=255) gtgCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCa > 1:134587/1‑90 (MQ=255) gtgCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCa > 2:405283/1‑90 (MQ=255) gCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACt > 2:233751/1‑90 (MQ=255) tttGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTccc > 2:356895/1‑90 (MQ=255) | GATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCC > NZ_CP009273/510002‑510158 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |