| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,213,865 | 0 | G | T | 61.5% | ‑3.2 / 13.0 | 13 | S361I (AGT→ATT) | pdeG | cyclic diguanylate phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base G (4/1); new base T (0/8); total (4/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.95e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCA > NZ_CP009273/1213781‑1213943 | aaGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTa > 2:58412/1‑90 (MQ=255) tttCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACccgc > 1:547562/1‑90 (MQ=255) cATTTTGAATTTATTTTTAGCGTTTCGTATTTTTTTTCGCCGACGTTTGTCGACGATTTTTTAATTTTCCGTGACATTTTTCCCcgccgc < 1:262396/90‑1 (MQ=255) tatttttAGCTTTTCGTATTTTTTTTCGCCGCTGTTTGTCGACGAGTTTTTAATTTTCCGTGACATTTTTCCCCGCCGCGATTTAAACCt < 1:58412/87‑1 (MQ=255) atttttAGCTCTTCGCTTTTTTTTCCGCCGACGTTTGTCGAGGAGTTTTTAATTTTCCGTGACATTTTTCCCCGCCGCGATTTAAACCtt < 1:465166/88‑1 (MQ=255) tCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTc > 1:153493/1‑90 (MQ=255) tccGCCGACGTTTGTCGACGAGTTTTTAATTTTCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGa < 2:140759/88‑1 (MQ=255) cGCCGACTTTTGTCGAGGATTTTTTAATTTTCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGc < 1:172021/90‑1 (MQ=255) cGCCGACTTTTGTCGACGAGTTTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGc < 2:228023/90‑1 (MQ=255) ttttGTCGACGATTTTTTAATTTTCCGTGACTTTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGaa < 1:237267/89‑1 (MQ=255) ttcccGTAACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCa < 2:393971/87‑1 (MQ=255) ccGTGACATTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGc < 1:192764/59‑1 (MQ=255) ccGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGc > 2:192764/1‑59 (MQ=255) | AAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTTCCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTCTGGTTCA > NZ_CP009273/1213781‑1213943 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |