Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,927,6610AT54.5% 1.0 / 12.9 11S401T (TCA→ACA) eddphosphogluconate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base T (0/6);  total (3/8)
Fisher's exact test for biased strand distribution p-value = 6.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGGTTACCGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGAC  >  NZ_CP009273/1927582‑1927728
                                                                                |                                                                   
aGGTTACCGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGcc                                                           <  1:392924/90‑1 (MQ=255)
      ccGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTcc                                                     >  1:568525/1‑90 (MQ=255)
                     ggcccACCAATGAGGAAATAACGGTTTTTCCAAGGAGGCAACCCCATTGTTGTAGGGTTTTTTTCCCCCCCTTTCCCGCCAGTCCAGTTc                                       <  1:409877/88‑1 (MQ=255)
                     gTCCCACC‑ATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCa                                      >  1:26109/1‑90 (MQ=255)
                         cccc‑aTGATGAGAGAAGGTTTTTTCGAAGGAAGCAATCACTTTGCTGTCGATTGTTTTTCCCCCCCCTTCCCGCCAGTCCAGTTCACCat                                  <  1:610273/88‑1 (MQ=255)
                                     gagaAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCAt                       <  2:26109/90‑1 (MQ=255)
                                          agtttgttCGAAGGAAGCGATCACATTGCTGTCGAGTGTTTTTTCCCCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTc                  <  2:558146/87‑1 (MQ=255)
                                            gtttttCAAAGAAAGCGATCACTTTGCTGTCGAGTGTTTTTCCCCCCCCTCCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCaa                <  2:568525/90‑1 (MQ=255)
                                            gttgttCAAAGGAGGCGATCACTTTGCTGTGGGGTGTTTTTCCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCaa                <  1:263889/90‑1 (MQ=255)
                                               gttCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAggg             >  2:481441/1‑90 (MQ=255)
                                                          gCGACCACATTGCTGTCGAGTGTTTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGagac  <  2:215742/90‑1 (MQ=255)
                                                                                |                                                                   
AGGTTACCGCTTAACACTTTTGTCCCACC‑ATGATGAGAGAAAGGTTGTTCGAAGGAAGCGATCACATTGCTGTCGAGTGATTTTTCCGCCCCTTCCCGCCAGTCCAGTTCACCATTATTCAGCCATGGTTCAAGGGTATAACGAGAC  >  NZ_CP009273/1927582‑1927728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑