| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,852,466 | 0 | T | G | 60.0% | ‑2.8 / 12.4 | 20 | V672G (GTG→GGG) | mutS | DNA mismatch repair protein MutS |
| Reads supporting (aligned to +/- strand): ref base T (7/1); new base G (0/12); total (7/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.03e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.43e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCT > NZ_CP009273/2852398‑2852555 | tATCGATCGCATCTTTACCCGCGTAGGCTCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCaa > 1:531837/1‑90 (MQ=255) cATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCaa > 1:21026/1‑81 (MQ=255) cATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGGGGAGATGACTGAAACCGCCaa < 2:21026/81‑1 (MQ=255) tccccGCGGGGGCGCGGCAGATGCCCGGGCGCCGGGGCCCTCACCTTTTAGGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGc < 1:266731/88‑1 (MQ=255) tccccGCGGGGGCGCGGAAGATGACCGGGCGTCCGGGCCCCCACCCTTTAGGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGc < 1:477637/88‑1 (MQ=255) tccccGAGTAGCCCCGGCAGACGGCCGGCGGTCCGGGCGCTCAACTTTTTTGGGGGAGAGGCCGAAACCCGCCAATATTTTACATAACGc < 2:531837/88‑1 (MQ=255) cgcgTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCAcc > 2:97681/1‑90 (MQ=255) cgcgTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCAcc > 1:169109/1‑90 (MQ=255) gggCGCGGAAGATGCCCGGGCGCCGGGGCCCTAACCCTTTAGGGGGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATa < 2:446241/89‑1 (MQ=255) aGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATa > 1:486803/1‑90 (MQ=255) ggCGCGGCAGATGCCCGGGCGTCCGGGCGCTCAACCTTTAGGGGGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATAc < 2:169109/90‑1 (MQ=255) ggaaGATGCCCGGGCGCCCGGGCCCTCACCTTTTGTGGGGGAGATGATTAAACCCGCCAATATTTTACATAACGCCACCGAATACAGTCt < 1:586052/87‑1 (MQ=255) ggCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCt > 2:158574/1‑90 (MQ=255) ggCAGAGGCCCTGGCGCCCGGGCCCCCAACTTTTAGGGGGGAGATGATTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCt < 1:568272/90‑1 (MQ=255) gCAGATGCCCTGGCGCCGGGGCCCTCACCCTTTAGGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTg < 1:263616/90‑1 (MQ=255) cAGATGCCCGGGCGCCCGGGCCCTCACCCTTTAGGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTgg < 1:529152/90‑1 (MQ=255) cAGATGCCCGGGCGCCCGGGCCCCCACCTTTTAGGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTgg < 1:554948/90‑1 (MQ=255) gggCGCTCAACTTTTAGGGTGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATc < 2:566310/90‑1 (MQ=255) gggCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATc > 2:112830/1‑90 (MQ=255) ttAGGGGGGAGATAACGAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGt < 1:301801/90‑1 (MQ=255) tggAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCt > 2:441529/1‑90 (MQ=255) | TATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACCTTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCT > NZ_CP009273/2852398‑2852555 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |