| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,955,854 | 0 | T | G | 57.1% | 5.5 / 10.8 | 14 | S90S (TCA→TCC) | ppdC | prepilin‑type N‑terminal cleavage/methylation domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (4/2); new base G (8/0); total (12/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.11e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGC > NZ_CP009273/2955781‑2955940 | ttAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGGGAAAATAGCGTAAAGc > 2:285182/1‑90 (MQ=255)ttAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGGCATCTCGCCTTCTCTGCCCCCGGGGGGAACTAGCGTAACGc > 1:474199/1‑90 (MQ=255) aaCATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTg < 1:285182/90‑1 (MQ=255) aGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGGGAAACTAGCGTAACGCTGATGCt > 2:245847/1‑90 (MQ=255) aGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCCGGGGAAACTAGCGTAACGCTGATGCt > 1:225944/1‑90 (MQ=255) aGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGGCATCTCCCCCTCTCTGCCCCCCGGGGAAACTAGCGTAACGCTGATGCt > 1:481234/1‑90 (MQ=255) tCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTTCCCCCCGGGGAAACTAGCGTAACGCTGATGCTGAcaca > 1:293454/1‑90 (MQ=255) actGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGGGAAACTAGCGGAACGCTGATGCTGACACATccccccc > 1:456184/1‑89 (MQ=255) gACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCg > 2:94685/1‑90 (MQ=255) aaTGCAGGCGGGTCATCTCGCCTTTTCTGCCCCCGGGGGAAAATAGCGTAACGCTGATGCTGGCCCATCCCCCCTGCCATGTCTGCATTc > 1:274641/1‑90 (MQ=255) ggCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCCGCGATGTCTGCATTCGGTTGa > 2:510491/1‑90 (MQ=255) gTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGc < 1:227478/49‑1 (MQ=255) gTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGc > 2:227478/1‑49 (MQ=255) gggTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATcgc > 2:41917/1‑90 (MQ=255) | TTAACATAGCGGCTCCTGACTACTGACGATTCGGGCAATGCAGGCGGGTCATCTCGCCTTCTCTGCCCCCGGGTGAAACTAGCGTAACGCTGATGCTGACACATCCCGCCTGCGATGTCTGCATTCGGTTGACCTGCCAGTTGGCAGGTGGCGAAATCGC > NZ_CP009273/2955781‑2955940 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |