| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,126,661 | 0 | T | G | 58.3% | 0.4 / 10.7 | 12 | H219P (CAC→CCC) | yghS | dTMP kinase |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (6/1); total (6/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGG > NZ_CP009273/3126583‑3126747 | tcCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATcc < 1:348180/90‑1 (MQ=255) cATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAgg < 2:424385/90‑1 (MQ=255) gATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATCCAGTTCAAGGATCTTTtcg > 2:9507/1‑90 (MQ=255) tCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGGGCCCACCCAGTTCAAGGGTGTTTTCGcc > 1:591471/1‑90 (MQ=255) gcgcGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATcc < 1:339897/62‑1 (MQ=255) gcgcGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCATcc > 2:339897/1‑62 (MQ=255) ggggcgTGAATTGCGCGTAGTGACCCTTGCAGATTTTCAGGGGGGGGGGGTCCCCCCTCCAGTTCAAGGATTTTTGCGCCATTGAATGTc < 1:248405/88‑1 (MQ=255) gcgTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGGCGCCCATCCCGTTTAAGGATCTTTTCGCCCTTGAATGTCAGt > 2:351901/1‑90 (MQ=255) tGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTgc < 2:621243/90‑1 (MQ=255) cgcgTAGTGACGCTTGCAGAATTTCATCGGCGGGGGGTCGCCCCTCCCCTTCAAGGATCTTTGCGCCCTTTAATGTCAGTTgcggcggga > 2:390637/1‑87 (MQ=255) tCATCGGCGGGGGGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCt > 2:151486/1‑90 (MQ=255) gggTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTtgg < 2:348018/90‑1 (MQ=255) | TCCCCTATCCATTTTGGTGATCAGGAAAGCGCGGCGTGAATTGCGCGTAGTGACGCTTGCAGAATTTCATCGGCGGGGTGTCGCCCATCCAGTTCAAGGATCTTTGCGCCATTGAATGTCAGTTGCGGCGTAACGGCGATTTTTTCCTGTAACGCTGCCAGTTGG > NZ_CP009273/3126583‑3126747 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |