| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,659,062 | 0 | C | A | 56.0% | 8.1 / 37.7 | 25 | A37S (GCG→TCG) | gadX | acid resistance transcriptional activator GadX |
| Reads supporting (aligned to +/- strand): ref base C (7/4); new base A (0/14); total (7/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.86e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.62e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGC > NZ_CP009273/3658975‑3659134 | gCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCaa > 2:280270/1‑90 (MQ=255)gCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCaa < 1:593291/90‑1 (MQ=255) tCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCaaaaa > 1:480437/1‑90 (MQ=255) gggAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAAcc > 1:207542/1‑90 (MQ=255) gggAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAAcc > 1:405055/1‑90 (MQ=255) aaatGAAAGCGTGTCCCTTGACAAAAAAAAAAAATTTCC‑AACCCACTTATCTACTAGATTTTGGCTTGCACCCGAAAAACCCAGGCCAcc < 1:478970/89‑1 (MQ=255) aatGAAAGCGTGTCCTTTAACACAAATAAAAATTTTTC‑AACACCCTTATCTCCTCAATTTTGGCTTGCACCCGAAAAACCCAGGTCACCg < 1:545602/90‑1 (MQ=255) aatGAAAGCGTGTCCCTTGACACAAATAAAAAATTTTC‑AACACCCTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCg < 2:79716/90‑1 (MQ=255) cacaAATAAAAATTTTTC‑AACACACTTATCTACTCAAATTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTc < 2:222706/90‑1 (MQ=255) cacaAATAAAAATTTTCC‑AACACTTTTATCTCCTCGATTTTGGTTTGCACCCGCAAAACCCAGGTCACCGCCATTAAAATAGCGATATTc < 2:377044/90‑1 (MQ=255) cacaAATAAAAAATTTTA‑AACACATTTATCTCCTCGATTTTGGCTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTc < 2:568726/90‑1 (MQ=255) aaTACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCa > 2:252524/1‑90 (MQ=255) tACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCAtt < 2:340362/90‑1 (MQ=255) aaaaTTTTCAAACACATTTATCTCCTCGATTTTGGCTTGCACCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTaa < 2:561275/90‑1 (MQ=255) tcc‑AACCCATTTATCTACTAAATTTTGGTTTCCACCCCAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGt < 1:568626/88‑1 (MQ=255) taaaCACCTTTATCTCCTCGATTTTGGCTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg < 2:304249/88‑1 (MQ=255) c‑AACCCCCTTATCTACACAATTTTGGCTTCCACCCAAAAAAACCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTga < 1:506966/90‑1 (MQ=255) aaCCCACTTATCTCCTCGACTTTGGCTTTCAACCAAAAACACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTgag < 1:280270/90‑1 (MQ=255) aaCACATTTATCTACTCAATTTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTgag < 2:586451/90‑1 (MQ=255) ccacTTATCTCCTCGATTTTGGCTTCCACCCGAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAat < 2:207542/89‑1 (MQ=255) acacTTATCTACTCGAATTTGGCTTGCATCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAat < 2:260748/90‑1 (MQ=255) tctcCTCAATTTGGGCTTCCACCCAAAAAACCCAGGCCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTa < 2:420185/90‑1 (MQ=255) cTCAAATTTGTTTTCCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTc < 1:166675/90‑1 (MQ=255) cGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCtt > 1:649700/1‑90 (MQ=255) aaTTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGACTTGc > 1:511392/1‑90 (MQ=255) | GCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTC‑AACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGC > NZ_CP009273/3658975‑3659134 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |